ORAI2 modulates store-operated calcium entry and T cell-mediated immunity

Martin Vaeth, Jun Yang, Megumi Yamashita, Isabelle Zee, Miriam Eckstein, Camille Knosp, Ulrike Kaufmann, Peter Karoly Jani, Rodrigo S. Lacruz, Veit Flockerzi, I. Kacskovics, Murali Prakriya, Stefan Feske

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Store-operated Ca2+ entry (SOCE) through Ca2+ release-activated Ca2+ (CRAC) channels is critical for lymphocyte function and immune responses. CRAC channels are hexamers of ORAI proteins that form the channel pore, but the contributions of individual ORAI homologues to CRAC channel function are not well understood. Here we show that deletion of Orai1 reduces, whereas deletion of Orai2 increases, SOCE in mouse T cells. These distinct effects are due to the ability of ORAI2 to form heteromeric channels with ORAI1 and to attenuate CRAC channel function. The combined deletion of Orai1 and Orai2 abolishes SOCE and strongly impairs Tcell function. In vivo, Orai1/Orai2 double-deficient mice have impaired T cell-dependent antiviral immune responses, and are protected from T cell-mediated autoimmunity and alloimmunity in models of colitis and graft-versus-host disease. Our study demonstrates that ORAI1 and ORAI2 form heteromeric CRAC channels, in which ORAI2 fine-tunes the magnitude of SOCE to modulate immune responses.

Original languageEnglish
Article number14714
JournalNature Communications
Volume8
DOIs
Publication statusPublished - Mar 15 2017

Fingerprint

T-cells
immunity
entry
Cellular Immunity
calcium
Calcium
T-Lymphocytes
deletion
Graft vs Host Disease
Colitis
Autoimmunity
Antiviral Agents
Lymphocytes
mice
Grafts
lymphocytes
Proteins
proteins
porosity

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Vaeth, M., Yang, J., Yamashita, M., Zee, I., Eckstein, M., Knosp, C., ... Feske, S. (2017). ORAI2 modulates store-operated calcium entry and T cell-mediated immunity. Nature Communications, 8, [14714]. https://doi.org/10.1038/ncomms14714

ORAI2 modulates store-operated calcium entry and T cell-mediated immunity. / Vaeth, Martin; Yang, Jun; Yamashita, Megumi; Zee, Isabelle; Eckstein, Miriam; Knosp, Camille; Kaufmann, Ulrike; Jani, Peter Karoly; Lacruz, Rodrigo S.; Flockerzi, Veit; Kacskovics, I.; Prakriya, Murali; Feske, Stefan.

In: Nature Communications, Vol. 8, 14714, 15.03.2017.

Research output: Contribution to journalArticle

Vaeth, M, Yang, J, Yamashita, M, Zee, I, Eckstein, M, Knosp, C, Kaufmann, U, Jani, PK, Lacruz, RS, Flockerzi, V, Kacskovics, I, Prakriya, M & Feske, S 2017, 'ORAI2 modulates store-operated calcium entry and T cell-mediated immunity', Nature Communications, vol. 8, 14714. https://doi.org/10.1038/ncomms14714
Vaeth M, Yang J, Yamashita M, Zee I, Eckstein M, Knosp C et al. ORAI2 modulates store-operated calcium entry and T cell-mediated immunity. Nature Communications. 2017 Mar 15;8. 14714. https://doi.org/10.1038/ncomms14714
Vaeth, Martin ; Yang, Jun ; Yamashita, Megumi ; Zee, Isabelle ; Eckstein, Miriam ; Knosp, Camille ; Kaufmann, Ulrike ; Jani, Peter Karoly ; Lacruz, Rodrigo S. ; Flockerzi, Veit ; Kacskovics, I. ; Prakriya, Murali ; Feske, Stefan. / ORAI2 modulates store-operated calcium entry and T cell-mediated immunity. In: Nature Communications. 2017 ; Vol. 8.
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