Optimum yield of pyridosine and furosine originating from maillard reactions monitored by ion-exchange chromatography

I. Molnár-perl, M. Pintér-szakács, R. Wittmann, M. Reutter, K. Eichner

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

An improved ion-exchange chromatographic method for rapid separation and quantitative estimation of deoxyfructosyllysines and their hydrolysis products (pyridosine and furosine) is described. The proposed elution procedure has good reproducibility; the optimum hydrolysis conditions for the highest molar ratio of pyridosine and furosine are given. The furosine/pyridosine molar ratios obtained by ⌈dhydrolysis" of model fructosyllysine as well as of natural protein matrices (soya beans, tomato powder, barley and malts) are in good agreement and without exception > 1. The smallest amount of pyridosine determined was 0.009% (w/w). The relationship between the compounds pyridosine, furosine, lysine and carbohydrate units present in the parent moiety is discussed.

Original languageEnglish
Pages (from-to)311-320
Number of pages10
JournalJournal of Chromatography A
Volume361
Issue numberC
DOIs
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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