Optimizing the sample size and the retention parameters to achieve maximum production rates for enantiomers in chiral chromatography

S. C. Jacobson, A. Felinger, G. Guiochon

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The optimum experimental conditions (sample size and mobile phase composition) are calculated for maximum production rate of either one of two enantiomers contained in feeds of different compositions (1/1, 1/10, and 10/1). The products are obtained at 99% purity. The calculations use the equilibrium-dispersive model of chromatography and the equilibrium isotherms determined experimentally from the rear, diffuse boundary of overloaded elution profiles. The production rate measured experimentally under the optimum conditions calculated agree within 4% of the calculated values. There is an optimum value for the retention factor which is higher than predicted by a model assuming constant separation factor, because both separation factor and retention decrease with increasing organic solvent concentration in the mobile phase.

Original languageEnglish
Pages (from-to)1210-1217
Number of pages8
JournalBiotechnology and Bioengineering
Volume40
Issue number10
DOIs
Publication statusPublished - Dec 5 1992

Fingerprint

Enantiomers
Chromatography
Sample Size
Phase composition
Organic solvents
Isotherms
Chemical analysis

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Optimizing the sample size and the retention parameters to achieve maximum production rates for enantiomers in chiral chromatography. / Jacobson, S. C.; Felinger, A.; Guiochon, G.

In: Biotechnology and Bioengineering, Vol. 40, No. 10, 05.12.1992, p. 1210-1217.

Research output: Contribution to journalArticle

@article{4ad2df8bcdda40449fd42ec42b35aad2,
title = "Optimizing the sample size and the retention parameters to achieve maximum production rates for enantiomers in chiral chromatography",
abstract = "The optimum experimental conditions (sample size and mobile phase composition) are calculated for maximum production rate of either one of two enantiomers contained in feeds of different compositions (1/1, 1/10, and 10/1). The products are obtained at 99{\%} purity. The calculations use the equilibrium-dispersive model of chromatography and the equilibrium isotherms determined experimentally from the rear, diffuse boundary of overloaded elution profiles. The production rate measured experimentally under the optimum conditions calculated agree within 4{\%} of the calculated values. There is an optimum value for the retention factor which is higher than predicted by a model assuming constant separation factor, because both separation factor and retention decrease with increasing organic solvent concentration in the mobile phase.",
author = "Jacobson, {S. C.} and A. Felinger and G. Guiochon",
year = "1992",
month = "12",
day = "5",
doi = "10.1002/bit.260401011",
language = "English",
volume = "40",
pages = "1210--1217",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "10",

}

TY - JOUR

T1 - Optimizing the sample size and the retention parameters to achieve maximum production rates for enantiomers in chiral chromatography

AU - Jacobson, S. C.

AU - Felinger, A.

AU - Guiochon, G.

PY - 1992/12/5

Y1 - 1992/12/5

N2 - The optimum experimental conditions (sample size and mobile phase composition) are calculated for maximum production rate of either one of two enantiomers contained in feeds of different compositions (1/1, 1/10, and 10/1). The products are obtained at 99% purity. The calculations use the equilibrium-dispersive model of chromatography and the equilibrium isotherms determined experimentally from the rear, diffuse boundary of overloaded elution profiles. The production rate measured experimentally under the optimum conditions calculated agree within 4% of the calculated values. There is an optimum value for the retention factor which is higher than predicted by a model assuming constant separation factor, because both separation factor and retention decrease with increasing organic solvent concentration in the mobile phase.

AB - The optimum experimental conditions (sample size and mobile phase composition) are calculated for maximum production rate of either one of two enantiomers contained in feeds of different compositions (1/1, 1/10, and 10/1). The products are obtained at 99% purity. The calculations use the equilibrium-dispersive model of chromatography and the equilibrium isotherms determined experimentally from the rear, diffuse boundary of overloaded elution profiles. The production rate measured experimentally under the optimum conditions calculated agree within 4% of the calculated values. There is an optimum value for the retention factor which is higher than predicted by a model assuming constant separation factor, because both separation factor and retention decrease with increasing organic solvent concentration in the mobile phase.

UR - http://www.scopus.com/inward/record.url?scp=0027113005&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027113005&partnerID=8YFLogxK

U2 - 10.1002/bit.260401011

DO - 10.1002/bit.260401011

M3 - Article

VL - 40

SP - 1210

EP - 1217

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 10

ER -