Optimization of phytase production by solid substrate fermentation

B. Bogar, G. Szakacs, J. C. Linden, A. Pandey, R. P. Tengerdy

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The production of phytase by three feed-grade filamentous fungi (Aspergillus ficuurn NRRL 3135, Mucor racemosus NRRL 1994 and Rhizopus oligosporus NRRL 5905) on four commonly used natural feed ingredients (canola meal, cracked corn, soybean meal, wheat bran) was studied in solid substrate fermentation (SSF). A. ficuum NRRL 3135 had the highest yield [15 IU phytase activity/g dry matter (DM)] on wheat bran. By optimizing the supplementation of wheat bran with starch and (NH4)2SO4, phytase production increased to 25 IU/g DM. Optimization was carried out by Plackett-Burman and central composite experimental designs. Using optimized medium, phytase, phosphatase, alpha-amylase and xylanase production by A. ficuum NRRL 3135 was studied in Erlenmeyer flask and tray SSF. By scaling up SSF from flasks to stationary trays, activities of 20 IU phytase activity/g DM were reproducibly obtained.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
JournalJournal of Industrial Microbiology and Biotechnology
Volume30
Issue number3
DOIs
Publication statusPublished - Mar 1 2003

    Fingerprint

Keywords

  • Aspergillus ficuum
  • Optimization
  • Phytase
  • Solid substrate fermentation
  • Statistical experimental design

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this