Optimization of operating parameters in overpressured thin-layer chromatography

Research output: Contribution to journalArticle

59 Citations (Scopus)


By adjusting the solvent by means of a pump system in overpressured thin-layer chromatography using a pressurized ultramicro (PUM) chamber it is possible to separate substances with optional development distances. In the PUM chamber the external pressure on the flexible cover membrane must always be higher than the input pressure of the solvent. The input pressure of the solvent increases linearly with increasing solvent migration distance. An increase in the solvent flow velocity always results in higher input pressures, which must be taken into account by choosing an appropriate external pressure on the membrane. The number of theoretical plates and the separation numbers obtained with a PUM chamber of the linear type on fine-particle sorbent layers are also better with longer solvent migration distances than in a normal TLC chamber. The advantages and the necessity for development with a longer migration distance are demonstrated with the example of the separation of amino acids on a fine-particle silica gel chromatoplate.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalJournal of Chromatography A
Issue number1
Publication statusPublished - Jun 26 1981

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Optimization of operating parameters in overpressured thin-layer chromatography'. Together they form a unique fingerprint.

  • Cite this