Optimization of growth conditions for test fungus cultures used in direct bioautographic TLC detection. 3. Test fungus

Candida albicans

Sándor Nagy, Béla Kocsis, T. Kőszegi, Lajos Botz

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Optimum conditions have been established for culture of the fungus Candida albicans (ATCC 90028) for microbial detection of zones in direct bioautographic TLC. Bioluminescent ATP assay is a highly sensitive method for optimizing the viability of Candida albicans test fungus in bioautographic TLC detection. A suspension of microbes (OD 600nm = 0.5-0.7, in Mueller-Hinton broth with 5% glucose) in the log phase of growth can be used for dipping TLC plates. On the basis of our results with Candida albicans, we can differentiate between microbiostatic (bacteriostatic or fungistatic) and microbiocidal (bactericidal or fungicidal) effects on TLC plates. Our micrographs clearly show the borders of inhibition zones in bioautograms. This technique leads to new possibilities in studies of the interactions between microbes and antimicrobial compounds on bioautographic silica gel TLC plates by using scanning electron microscopy. In this paper we describe an optimization procedure for bioautographic TLC detection.

Original languageEnglish
Pages (from-to)385-389
Number of pages5
JournalJournal of Planar Chromatography - Modern TLC
Volume20
Issue number5
DOIs
Publication statusPublished - Oct 2007

Fingerprint

Candida
Fungi
Candida albicans
Growth
Luminescent Measurements
Silica Gel
Electron Scanning Microscopy
Assays
Suspensions
Adenosine Triphosphate
Glucose
Scanning electron microscopy

Keywords

  • Bioautography
  • Bioluminescent ATP assay
  • Candida albicans
  • Direct bioautography
  • Fungistatic and fungicidal antibiotics
  • Scanning electron microscopy
  • TLC

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Optimization of growth conditions for test fungus cultures used in direct bioautographic TLC detection. 3. Test fungus : Candida albicans. / Nagy, Sándor; Kocsis, Béla; Kőszegi, T.; Botz, Lajos.

In: Journal of Planar Chromatography - Modern TLC, Vol. 20, No. 5, 10.2007, p. 385-389.

Research output: Contribution to journalArticle

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