Optimization of electroporation conditions for maize microspores

B. Obert, Z. Pónya, A. Pret'ová, B. Barnabás

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Microspore culture can be used for homozygous dihaploid plant production and the uninuclear origin of microspore-derived dihaploid plant is an excellent basis for plant transformation, as a plant with integrated foreign gene in a homozygous set can be obtained in a considerably short time. An electroporation method for delivery of foreign DNA into mechanically isolated microspores of maize (Zea mays L.) was developed. Different media for electroporation and different concentrations of plasmid DNA were tested. Physical factors affecting efficiency of electroporation (number of pulses, duration of the pulse, voltage, frequency) and plasmid DNA concentration were optimised and discussed, relating to microspore viability, induction of gametic embryogenesis after electroporation, and the number of GUS expressing microspores.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalMaydica
Volume49
Issue number1
Publication statusPublished - Jul 30 2004

Keywords

  • Androgenesis
  • Electroporation
  • Maize
  • Microspore culture
  • Plant transformation
  • Zea mays L.

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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  • Cite this

    Obert, B., Pónya, Z., Pret'ová, A., & Barnabás, B. (2004). Optimization of electroporation conditions for maize microspores. Maydica, 49(1), 15-19.