Optimization of desferrioxamine e production by streptomyces parvulus

Tamás Gáll, Gábor Lehoczki, Gyöngyi Gyémánt, Tamás Emri, Zsuzsa M. Szigeti, György Balla, Jó Zsef Balla, István Pócsi

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2 Citations (Scopus)

Abstract

Siderophores are produced by a number of microbes to capture iron with outstandingly high affinity, which property also generates biomedical and industrial interests. Desferrioxamine E (DFO-E) secreted by streptomycetes bacteria can be an ideal candidate for iron chelation therapy, which necessitates its costeffective production for in vitro and animal studies. This study focused on the optimization of DFO-E production by Streptomyces parvulus CBS548.68. Different combinations of various carbon and nitrogen sources as well as the addition of 3-morpholinopropane-1-sulfonic acid (MOPS) markedly affected DFO-E yields, which were attributed, at least in part, to the higher biomass productions found in MOPS-supplemented cultures. In MOPS-supplemented glucose and sodium glutamate medium, DFO-E productions as high as 2,009 ± 90 mg/l of culture medium were reached. High-performance liquid chromatography analysis demonstrated that a simple two-step purification process yielded DFO-E preparations with purities of ±97%. Matrix assisted laser desorption ionization-Time of flight mass spectrometry analysis showed that purified DFO-E always contained traces of desferrioxamine D2.

Original languageEnglish
Pages (from-to)475-489
Number of pages15
JournalActa microbiologica et immunologica Hungarica
Volume63
Issue number4
DOIs
Publication statusPublished - Dec 2016

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Keywords

  • Desferrioxamine E
  • MOPS
  • Na-glutamate
  • Streptomyces parvulus

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

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