Optimization of coprogen production in Neurospora crassa

Viktória Tóth, K. Antal, G. Gyémánt, M. Miskei, I. Pócsi, T. Emri

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Coprogen production of Neurospora crassa was dependent on glucose, aspartate and iron contents as well as on initial pH of the culture media. Surplus iron and acidic pH hindered the production of coprogen as well as the transcription of the sid1 gene (NCU07117) encoding putative L-ornithine-N 5-monooxygenase, the first enzyme in the coprogen biosynthetic pathway. High glucose (40 g/l) and aspartate (21 g/l) concentrations were beneficial for coprogen synthesis, but neither glucose nor aspartate affected the sid1 transcription. Moreover, efficient coprogen production was observed after glucose had been consumed, which suggested that N. crassa accumulated iron even in non-growing, carbon-starving cultures.

Original languageEnglish
Pages (from-to)321-328
Number of pages8
JournalActa Biologica Hungarica
Volume60
Issue number3
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Neurospora crassa
glucose
Aspartic Acid
Glucose
iron
Iron
Transcription
Gene encoding
Ornithine
Biosynthetic Pathways
Mixed Function Oxygenases
Culture Media
enzyme
Carbon
coprogen
gene
carbon
Enzymes
Genes

Keywords

  • Coprogen
  • L-ornithine-N-monooxygenase
  • Neurospora crassa
  • Response surface methodology
  • Siderophore

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Optimization of coprogen production in Neurospora crassa. / Tóth, Viktória; Antal, K.; Gyémánt, G.; Miskei, M.; Pócsi, I.; Emri, T.

In: Acta Biologica Hungarica, Vol. 60, No. 3, 09.2009, p. 321-328.

Research output: Contribution to journalArticle

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