Optimization of coprogen production in Neurospora crassa

Viktória Tóth, K. Antal, Gyöngyi Gyémánt, M. Miskei, I. Pócsi, T. Emri

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Coprogen production of Neurospora crassa was dependent on glucose, aspartate and iron contents as well as on initial pH of the culture media. Surplus iron and acidic pH hindered the production of coprogen as well as the transcription of the sid1 gene (NCU07117) encoding putative L-ornithine-N 5-monooxygenase, the first enzyme in the coprogen biosynthetic pathway. High glucose (40 g/l) and aspartate (21 g/l) concentrations were beneficial for coprogen synthesis, but neither glucose nor aspartate affected the sid1 transcription. Moreover, efficient coprogen production was observed after glucose had been consumed, which suggested that N. crassa accumulated iron even in non-growing, carbon-starving cultures.

Original languageEnglish
Pages (from-to)321-328
Number of pages8
JournalActa biologica Hungarica
Volume60
Issue number3
DOIs
Publication statusPublished - Sep 1 2009

Keywords

  • Coprogen
  • L-ornithine-N-monooxygenase
  • Neurospora crassa
  • Response surface methodology
  • Siderophore

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

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