Optimisation of sodium and potassium concentrations and pH in the artificial seminal plasma of common carp Cyprinus carpio L.

Beata Irena Cejko, A. Horváth, Timea Kollár, Eszter Kása, Jelena Lujić, Zoran Marinović, B. Urbányi, Radosław Kajetan Kowalski

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The effect of sodium and potassium concentrations as well as optimal pH on the motility of common carp Cyprinus carpio L. sperm during short-term storage in artificial seminal plasma (ASP) was investigated. Sperm was collected from individual males (n = 5) and each sample diluted tenfold (1:9) in ASP (sperm:extender) containing 2 mM CaCl2, 1 mM Mg2SO4 and 20 mM Tris at pH 8.0 and supplemented by the following concentrations of sodium and potassium (mM/mM): 0/150, 20/130, 40/110, 75/75, 110/40, 130/20 and 150/0. The osmolality of all ASP variants was set at 310 mOsm kg−1. Sperm motility was measured using a CASA system during 72 h of storage. Immediately after dilution, sperm motility was high (90%) both in each variant and in the control group (fresh sperm). After 72-h storage, the highest sperm motility was noted in ASP containing 110 mM NaCl and 40 mM KCl. No differences were found in the motility of samples preserved within the pH range of 7.0–9.0. Our data suggest that for the short-term storage of common carp sperm, whereas the pH of the solution does not play a crucial role, a specific potassium concentration of around 40 mM is required.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalFish Physiology and Biochemistry
DOIs
Publication statusAccepted/In press - Mar 21 2018

Fingerprint

Carps
seminal plasma
Semen
Cyprinus carpio
sperm
Spermatozoa
Potassium
potassium
Sodium
spermatozoa
sodium
Sperm Motility
motility
Plasmas
plasma
sperm motility
Dilution
osmolality
Osmolar Concentration
sampling

Keywords

  • Artificial seminal plasma
  • Chilled storage
  • Common carp
  • pH
  • Potassium
  • Sodium
  • Sperm preservation

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Aquatic Science

Cite this

Optimisation of sodium and potassium concentrations and pH in the artificial seminal plasma of common carp Cyprinus carpio L. / Cejko, Beata Irena; Horváth, A.; Kollár, Timea; Kása, Eszter; Lujić, Jelena; Marinović, Zoran; Urbányi, B.; Kowalski, Radosław Kajetan.

In: Fish Physiology and Biochemistry, 21.03.2018, p. 1-8.

Research output: Contribution to journalArticle

Cejko, Beata Irena ; Horváth, A. ; Kollár, Timea ; Kása, Eszter ; Lujić, Jelena ; Marinović, Zoran ; Urbányi, B. ; Kowalski, Radosław Kajetan. / Optimisation of sodium and potassium concentrations and pH in the artificial seminal plasma of common carp Cyprinus carpio L. In: Fish Physiology and Biochemistry. 2018 ; pp. 1-8.
@article{0b50eaa3df83447a8b657beea665485e,
title = "Optimisation of sodium and potassium concentrations and pH in the artificial seminal plasma of common carp Cyprinus carpio L.",
abstract = "The effect of sodium and potassium concentrations as well as optimal pH on the motility of common carp Cyprinus carpio L. sperm during short-term storage in artificial seminal plasma (ASP) was investigated. Sperm was collected from individual males (n = 5) and each sample diluted tenfold (1:9) in ASP (sperm:extender) containing 2 mM CaCl2, 1 mM Mg2SO4 and 20 mM Tris at pH 8.0 and supplemented by the following concentrations of sodium and potassium (mM/mM): 0/150, 20/130, 40/110, 75/75, 110/40, 130/20 and 150/0. The osmolality of all ASP variants was set at 310 mOsm kg−1. Sperm motility was measured using a CASA system during 72 h of storage. Immediately after dilution, sperm motility was high (90{\%}) both in each variant and in the control group (fresh sperm). After 72-h storage, the highest sperm motility was noted in ASP containing 110 mM NaCl and 40 mM KCl. No differences were found in the motility of samples preserved within the pH range of 7.0–9.0. Our data suggest that for the short-term storage of common carp sperm, whereas the pH of the solution does not play a crucial role, a specific potassium concentration of around 40 mM is required.",
keywords = "Artificial seminal plasma, Chilled storage, Common carp, pH, Potassium, Sodium, Sperm preservation",
author = "Cejko, {Beata Irena} and A. Horv{\'a}th and Timea Koll{\'a}r and Eszter K{\'a}sa and Jelena Lujić and Zoran Marinović and B. Urb{\'a}nyi and Kowalski, {Radosław Kajetan}",
year = "2018",
month = "3",
day = "21",
doi = "10.1007/s10695-018-0491-3",
language = "English",
pages = "1--8",
journal = "Fish Physiology and Biochemistry",
issn = "0920-1742",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Optimisation of sodium and potassium concentrations and pH in the artificial seminal plasma of common carp Cyprinus carpio L.

AU - Cejko, Beata Irena

AU - Horváth, A.

AU - Kollár, Timea

AU - Kása, Eszter

AU - Lujić, Jelena

AU - Marinović, Zoran

AU - Urbányi, B.

AU - Kowalski, Radosław Kajetan

PY - 2018/3/21

Y1 - 2018/3/21

N2 - The effect of sodium and potassium concentrations as well as optimal pH on the motility of common carp Cyprinus carpio L. sperm during short-term storage in artificial seminal plasma (ASP) was investigated. Sperm was collected from individual males (n = 5) and each sample diluted tenfold (1:9) in ASP (sperm:extender) containing 2 mM CaCl2, 1 mM Mg2SO4 and 20 mM Tris at pH 8.0 and supplemented by the following concentrations of sodium and potassium (mM/mM): 0/150, 20/130, 40/110, 75/75, 110/40, 130/20 and 150/0. The osmolality of all ASP variants was set at 310 mOsm kg−1. Sperm motility was measured using a CASA system during 72 h of storage. Immediately after dilution, sperm motility was high (90%) both in each variant and in the control group (fresh sperm). After 72-h storage, the highest sperm motility was noted in ASP containing 110 mM NaCl and 40 mM KCl. No differences were found in the motility of samples preserved within the pH range of 7.0–9.0. Our data suggest that for the short-term storage of common carp sperm, whereas the pH of the solution does not play a crucial role, a specific potassium concentration of around 40 mM is required.

AB - The effect of sodium and potassium concentrations as well as optimal pH on the motility of common carp Cyprinus carpio L. sperm during short-term storage in artificial seminal plasma (ASP) was investigated. Sperm was collected from individual males (n = 5) and each sample diluted tenfold (1:9) in ASP (sperm:extender) containing 2 mM CaCl2, 1 mM Mg2SO4 and 20 mM Tris at pH 8.0 and supplemented by the following concentrations of sodium and potassium (mM/mM): 0/150, 20/130, 40/110, 75/75, 110/40, 130/20 and 150/0. The osmolality of all ASP variants was set at 310 mOsm kg−1. Sperm motility was measured using a CASA system during 72 h of storage. Immediately after dilution, sperm motility was high (90%) both in each variant and in the control group (fresh sperm). After 72-h storage, the highest sperm motility was noted in ASP containing 110 mM NaCl and 40 mM KCl. No differences were found in the motility of samples preserved within the pH range of 7.0–9.0. Our data suggest that for the short-term storage of common carp sperm, whereas the pH of the solution does not play a crucial role, a specific potassium concentration of around 40 mM is required.

KW - Artificial seminal plasma

KW - Chilled storage

KW - Common carp

KW - pH

KW - Potassium

KW - Sodium

KW - Sperm preservation

UR - http://www.scopus.com/inward/record.url?scp=85046040704&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046040704&partnerID=8YFLogxK

U2 - 10.1007/s10695-018-0491-3

DO - 10.1007/s10695-018-0491-3

M3 - Article

C2 - 29560576

AN - SCOPUS:85046040704

SP - 1

EP - 8

JO - Fish Physiology and Biochemistry

JF - Fish Physiology and Biochemistry

SN - 0920-1742

ER -