Optimisation of Composition of Media for the Production of Amylolytic Enzymes by Thermomyces lanuginosus ATCC 34626

Q. Nguyen, Judit M. Rezessy-Szabó, Ágoston Hoschke

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The composition of media for the production of amylolytic enzymes by Thermomyces lanuginosus was optimised in different ways. Effects of various carbon and nitrogen sources were investigated. Thermomyces lanuginosus grown on starch, maltodextrin, dextrin, maltose, amylopectin, glucose and dextran substrates showed good α-amylase (92-125 U/mL) and glucoamylase (6-13 U/mL) activities. Among the tested nitrogen sources L-asparagine was the best one. The optimum pH of fermentation medium was found and fixed to 4.9 by using 100 mM citrate buffer for the production of amylolytic enzymes. Response Surface Method (RSM) was applied for searching the optimum concentration of components of media for amylolytic enzyme production. A second-order polynomial model was fitted at significance level 95 % (P

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalFood Technology and Biotechnology
Volume38
Issue number3
Publication statusPublished - Jul 2000

Fingerprint

Thermomyces lanuginosus
Enzymes
Nitrogen
enzymes
Chemical analysis
Amylopectins
Amylopectin
Maltose
Glucan 1,4-alpha-Glucosidase
glucan 1,4-alpha-glucosidase
dextrins
Amylases
Dextran
maltodextrins
Asparagine
amylopectin
Statistical Models
dextran
asparagine
nitrogen

Keywords

  • α-amylase
  • Fermentation
  • Glucoamylase
  • Response Surface Method (RSM)
  • Thermomyces lanuginosus

ASJC Scopus subject areas

  • Food Science
  • Biotechnology

Cite this

Optimisation of Composition of Media for the Production of Amylolytic Enzymes by Thermomyces lanuginosus ATCC 34626. / Nguyen, Q.; Rezessy-Szabó, Judit M.; Hoschke, Ágoston.

In: Food Technology and Biotechnology, Vol. 38, No. 3, 07.2000, p. 229-234.

Research output: Contribution to journalArticle

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