Optimal conditions for mycelial growth of Schizosaccharomyces japonicus cells in liquid medium: It enables the molecular investigation of dimorphism

László Papp, M. Sipiczki, I. Holb, I. Miklós

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The non-pathogenic dimorphic fission yeast, Schizosaccharomyces japonicus, could be a suitable model organism for investigation of the genetic background of mycelial growth, as it has a haploid chromosome set and its genome is sequenced. Since earlier results have suggested that its morphological transition required solid substrates, but molecular biological experiments would require hyphae production in a liquid medium, we wanted to find circumstances which would enable hyphae production in liquid media. Several external conditions were investigated, but the strongest inducer was fetal bovine serum (FBS). Its positive effect could be hampered by heat and was dependent on pH, temperature and concentration of the serum. Other protein-containing compounds, such as peptone and bovine serum albumin or amino acids, proved to be ineffective or weak. Generally, the uninduced and induced mycelial growth of Sz. japonicus could be improved by lower external pH and higher temperature.

Original languageEnglish
Pages (from-to)475-482
Number of pages8
JournalYeast
Volume31
Issue number12
DOIs
Publication statusPublished - Dec 1 2014

Fingerprint

Hyphae
Schizosaccharomyces
Peptones
Temperature
Haploidy
Liquids
Chromosomes
Growth
Bovine Serum Albumin
Serum
Yeast
Amino acids
Hot Temperature
Genes
Genome
Proteins
Amino Acids
Substrates
Experiments
Genetic Background

Keywords

  • Dimorphism
  • Hyphae
  • Mycelial growth
  • Schizosaccharomyces japonicus

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Medicine(all)

Cite this

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AU - Papp, László

AU - Sipiczki, M.

AU - Holb, I.

AU - Miklós, I.

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AB - The non-pathogenic dimorphic fission yeast, Schizosaccharomyces japonicus, could be a suitable model organism for investigation of the genetic background of mycelial growth, as it has a haploid chromosome set and its genome is sequenced. Since earlier results have suggested that its morphological transition required solid substrates, but molecular biological experiments would require hyphae production in a liquid medium, we wanted to find circumstances which would enable hyphae production in liquid media. Several external conditions were investigated, but the strongest inducer was fetal bovine serum (FBS). Its positive effect could be hampered by heat and was dependent on pH, temperature and concentration of the serum. Other protein-containing compounds, such as peptone and bovine serum albumin or amino acids, proved to be ineffective or weak. Generally, the uninduced and induced mycelial growth of Sz. japonicus could be improved by lower external pH and higher temperature.

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