Optical waveguide lightmode spectroscopy technique-based immunosensor development for aflatoxin B1 determination in spice paprika samples

Krisztina Majer-Baranyi, Zsolt Zalán, Mária Mörtl, Judit Juracsek, István Szendro, András Székács, Nóra Adányi

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13 Citations (Scopus)

Abstract

Optical waveguide lightmode spectroscopy (OWLS) technique has been applied to label-free detection of aflatoxin B1 in a competitive immunoassay format, with the aim to compare the analytical goodness of the developed OWLS immunosenor with HPLC and enzyme-linked immunosorbent assay (ELISA) methods for the detection of aflatoxin in spice paprika matrix. We have also assessed applicability of the QuEChERS method prior to ELISA measurements, and the results were compared to those obtained by traditional solvent extraction followed by immunoaffinity clean-up. The AFB1 content of sixty commercial spice paprika samples from different countries were measured with the developed and optimized OWLS immunosensor. Comparing the results from the indirect immunosensor to that obtained by HPLC or ELISA provided excellent correlation (with regression coefficients above 0.94) indicating that the competitive OWLS immunosensor has a potential for quick determination of aflatoxin B1 in paprika samples.

Original languageEnglish
Pages (from-to)972-977
Number of pages6
JournalFood Chemistry
Volume211
DOIs
Publication statusPublished - Nov 15 2016

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Keywords

  • Aflatoxin B1
  • ELISA
  • Immunosensor
  • OWLS
  • Spice paprika

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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