Optical waveguide-based biosensor for label-free monitoring of living cells

N. Orgovan, B. Szabó, R. Horváth

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Here, we briefly discuss the past, present, and possible future of label-free optical biosensors in cell research, especially focusing on the kinetic monitoring of cellular adhesion. Currently available optical biosensors possess outstanding potentials still not rightfully recognized and still waiting to be fully exploited in the field of cell science. Thus, during the description we give special emphasis to the advantages that the state-of-the-art optical cell-based biosensors possess as compared to microscopeor force measurement-based techniques widely used to characterize cell adhesion. To name here only a few, they enable label-free detection close to a planar sensor surface, have high sensitivity, and generate superior quality kinetic data. Such information-rich kinetic data, in turn, can be analyzed in-depth and comparatively. To exemplify the importance of in-depth kinetic analysis, we review a recent study, in which the Epic BenchTop high-throughput optical biosensor was used to measure the dependence of cancer cell adhesion kinetics on the surface density of integrin ligands. Based on the kinetic data, a model enabling the label-free determination of the dissociation constant of the adhesion ligands bound to their native cell membrane receptors has been constructed. As an outlook, the perspectives of the technology is briefly discussed.

Original languageEnglish
Title of host publicationNanobiosensors for Personalized and Onsite Biomedical Diagnosis
PublisherInstitution of Engineering and Technology
Pages165-180
Number of pages16
ISBN (Electronic)9781849199513
ISBN (Print)9781849199506
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Biosensing Techniques
Optical waveguides
Biosensors
Labels
Cells
Kinetics
Monitoring
Cell adhesion
Cell Adhesion
Adhesion
Ligands
Force measurement
Cell membranes
Integrins
Names
Throughput
Cell Membrane
Technology
Sensors
Research

Keywords

  • Adhesion ligand
  • Biomechanics
  • Biosensors
  • Cancer
  • Cancer cell adhesion kinetics
  • Cellular adhesion
  • Cellular biophysics
  • Epic benchtop
  • High-throughput optical biosensor
  • In-depth kinetic analysis
  • Information-rich kinetic data
  • Integrin ligand
  • Kinetic monitoring
  • Label-free detection
  • Label-free living-cell monitoring
  • Label-free optical biosensor
  • Microscopeor force measurement
  • Native cell membrane receptors
  • Optical sensors
  • Optical waveguide
  • Optical waveguides
  • Patient monitoring
  • Planar sensor surface
  • Proteins
  • Surface density

ASJC Scopus subject areas

  • Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Orgovan, N., Szabó, B., & Horváth, R. (2016). Optical waveguide-based biosensor for label-free monitoring of living cells. In Nanobiosensors for Personalized and Onsite Biomedical Diagnosis (pp. 165-180). Institution of Engineering and Technology. https://doi.org/10.1049/PBHE001E_ch9

Optical waveguide-based biosensor for label-free monitoring of living cells. / Orgovan, N.; Szabó, B.; Horváth, R.

Nanobiosensors for Personalized and Onsite Biomedical Diagnosis. Institution of Engineering and Technology, 2016. p. 165-180.

Research output: Chapter in Book/Report/Conference proceedingChapter

Orgovan, N, Szabó, B & Horváth, R 2016, Optical waveguide-based biosensor for label-free monitoring of living cells. in Nanobiosensors for Personalized and Onsite Biomedical Diagnosis. Institution of Engineering and Technology, pp. 165-180. https://doi.org/10.1049/PBHE001E_ch9
Orgovan N, Szabó B, Horváth R. Optical waveguide-based biosensor for label-free monitoring of living cells. In Nanobiosensors for Personalized and Onsite Biomedical Diagnosis. Institution of Engineering and Technology. 2016. p. 165-180 https://doi.org/10.1049/PBHE001E_ch9
Orgovan, N. ; Szabó, B. ; Horváth, R. / Optical waveguide-based biosensor for label-free monitoring of living cells. Nanobiosensors for Personalized and Onsite Biomedical Diagnosis. Institution of Engineering and Technology, 2016. pp. 165-180
@inbook{19a3263e915a45719eb6b3ffc39d9bd7,
title = "Optical waveguide-based biosensor for label-free monitoring of living cells",
abstract = "Here, we briefly discuss the past, present, and possible future of label-free optical biosensors in cell research, especially focusing on the kinetic monitoring of cellular adhesion. Currently available optical biosensors possess outstanding potentials still not rightfully recognized and still waiting to be fully exploited in the field of cell science. Thus, during the description we give special emphasis to the advantages that the state-of-the-art optical cell-based biosensors possess as compared to microscopeor force measurement-based techniques widely used to characterize cell adhesion. To name here only a few, they enable label-free detection close to a planar sensor surface, have high sensitivity, and generate superior quality kinetic data. Such information-rich kinetic data, in turn, can be analyzed in-depth and comparatively. To exemplify the importance of in-depth kinetic analysis, we review a recent study, in which the Epic BenchTop high-throughput optical biosensor was used to measure the dependence of cancer cell adhesion kinetics on the surface density of integrin ligands. Based on the kinetic data, a model enabling the label-free determination of the dissociation constant of the adhesion ligands bound to their native cell membrane receptors has been constructed. As an outlook, the perspectives of the technology is briefly discussed.",
keywords = "Adhesion ligand, Biomechanics, Biosensors, Cancer, Cancer cell adhesion kinetics, Cellular adhesion, Cellular biophysics, Epic benchtop, High-throughput optical biosensor, In-depth kinetic analysis, Information-rich kinetic data, Integrin ligand, Kinetic monitoring, Label-free detection, Label-free living-cell monitoring, Label-free optical biosensor, Microscopeor force measurement, Native cell membrane receptors, Optical sensors, Optical waveguide, Optical waveguides, Patient monitoring, Planar sensor surface, Proteins, Surface density",
author = "N. Orgovan and B. Szab{\'o} and R. Horv{\'a}th",
year = "2016",
month = "1",
day = "1",
doi = "10.1049/PBHE001E_ch9",
language = "English",
isbn = "9781849199506",
pages = "165--180",
booktitle = "Nanobiosensors for Personalized and Onsite Biomedical Diagnosis",
publisher = "Institution of Engineering and Technology",

}

TY - CHAP

T1 - Optical waveguide-based biosensor for label-free monitoring of living cells

AU - Orgovan, N.

AU - Szabó, B.

AU - Horváth, R.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Here, we briefly discuss the past, present, and possible future of label-free optical biosensors in cell research, especially focusing on the kinetic monitoring of cellular adhesion. Currently available optical biosensors possess outstanding potentials still not rightfully recognized and still waiting to be fully exploited in the field of cell science. Thus, during the description we give special emphasis to the advantages that the state-of-the-art optical cell-based biosensors possess as compared to microscopeor force measurement-based techniques widely used to characterize cell adhesion. To name here only a few, they enable label-free detection close to a planar sensor surface, have high sensitivity, and generate superior quality kinetic data. Such information-rich kinetic data, in turn, can be analyzed in-depth and comparatively. To exemplify the importance of in-depth kinetic analysis, we review a recent study, in which the Epic BenchTop high-throughput optical biosensor was used to measure the dependence of cancer cell adhesion kinetics on the surface density of integrin ligands. Based on the kinetic data, a model enabling the label-free determination of the dissociation constant of the adhesion ligands bound to their native cell membrane receptors has been constructed. As an outlook, the perspectives of the technology is briefly discussed.

AB - Here, we briefly discuss the past, present, and possible future of label-free optical biosensors in cell research, especially focusing on the kinetic monitoring of cellular adhesion. Currently available optical biosensors possess outstanding potentials still not rightfully recognized and still waiting to be fully exploited in the field of cell science. Thus, during the description we give special emphasis to the advantages that the state-of-the-art optical cell-based biosensors possess as compared to microscopeor force measurement-based techniques widely used to characterize cell adhesion. To name here only a few, they enable label-free detection close to a planar sensor surface, have high sensitivity, and generate superior quality kinetic data. Such information-rich kinetic data, in turn, can be analyzed in-depth and comparatively. To exemplify the importance of in-depth kinetic analysis, we review a recent study, in which the Epic BenchTop high-throughput optical biosensor was used to measure the dependence of cancer cell adhesion kinetics on the surface density of integrin ligands. Based on the kinetic data, a model enabling the label-free determination of the dissociation constant of the adhesion ligands bound to their native cell membrane receptors has been constructed. As an outlook, the perspectives of the technology is briefly discussed.

KW - Adhesion ligand

KW - Biomechanics

KW - Biosensors

KW - Cancer

KW - Cancer cell adhesion kinetics

KW - Cellular adhesion

KW - Cellular biophysics

KW - Epic benchtop

KW - High-throughput optical biosensor

KW - In-depth kinetic analysis

KW - Information-rich kinetic data

KW - Integrin ligand

KW - Kinetic monitoring

KW - Label-free detection

KW - Label-free living-cell monitoring

KW - Label-free optical biosensor

KW - Microscopeor force measurement

KW - Native cell membrane receptors

KW - Optical sensors

KW - Optical waveguide

KW - Optical waveguides

KW - Patient monitoring

KW - Planar sensor surface

KW - Proteins

KW - Surface density

UR - http://www.scopus.com/inward/record.url?scp=85014209465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85014209465&partnerID=8YFLogxK

U2 - 10.1049/PBHE001E_ch9

DO - 10.1049/PBHE001E_ch9

M3 - Chapter

AN - SCOPUS:85014209465

SN - 9781849199506

SP - 165

EP - 180

BT - Nanobiosensors for Personalized and Onsite Biomedical Diagnosis

PB - Institution of Engineering and Technology

ER -