Optical monitoring of stem cell-substratum interactions

Amirreza Aref, R. Horváth, James McColl, Jeremy J. Ramsden

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Modulation of the coupling of light into a waveguide via a grating, together with a novel approach to analyzing the data, is used to investigate the attachment of human embryonal carcinoma stem cells to three substrata: silica-titania (representative of artificial implants); poly-lysine (a commonly used laboratory cell culture substrate); and mucin (the coating of the mucosae). By considering both in-coupling peak width and position, the secretion of microexudate by the cells, the formation of filopodia, and the overall change in their shape (spreading) can be distinguished. This cannot be achieved by the conventional microscopic imaging approach. Moreover, we obtain the kinetics of these processes with excellent time resolution.

Original languageEnglish
Article number010501
JournalJournal of Biomedical Optics
Volume14
Issue number1
DOIs
Publication statusPublished - 2009

Fingerprint

stem cells
Mucins
Stem cells
Cell culture
Silicon Dioxide
Lysine
Waveguides
Titanium
Silica
Modulation
Imaging techniques
Coatings
secretions
Kinetics
Monitoring
lysine
Substrates
attachment
titanium
cancer

Keywords

  • fluorescence
  • microscopy
  • particles
  • synchroton radiation
  • tissues
  • x-rays

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Optical monitoring of stem cell-substratum interactions. / Aref, Amirreza; Horváth, R.; McColl, James; Ramsden, Jeremy J.

In: Journal of Biomedical Optics, Vol. 14, No. 1, 010501, 2009.

Research output: Contribution to journalArticle

Aref, Amirreza ; Horváth, R. ; McColl, James ; Ramsden, Jeremy J. / Optical monitoring of stem cell-substratum interactions. In: Journal of Biomedical Optics. 2009 ; Vol. 14, No. 1.
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