Optical biosensor for urea with improved response time

Barna Kovács, G. Nagy, Roland Dombi, K. Tóth

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

An optical biosensor for urea measurements was developed. The operation of the sensor is based on the well-known urease enzyme-catalyzed hydrolysis of urea. The ammonium ions liberated in the reaction are detected with an ion selective optode membrane containing nonactin as ion selective ionophore and ETH 5294 chromoionophore in a thin (1 μm) plasticized poly(vinylchloride) film. The basic sensing element was home made of a microscope glass slide, a HeNe laser light source, photodiode light detector and light in coupling, de-coupling elements. The transducer membrane and the enzyme containing reaction layer were sandwich-cast with spin coating onto the surface of the sensing slide. The attenuation of the laser light propagating inside the glass wave-guide was used as signal for urea measurements. With this arrangement membranes provided good sensitivity (0.05 absorption unit when going from 0.1 to 1 mM urea) and short (16-20 s) response time. Taking advantage on the improved response time, flow injection urea measurements were made in the 0.01-2 mM concentration range. Thirty sample/hour analysis-rate, good peak-to-peak reproducibility (RSD = 0.02) and recovery (95-104%) was achieved with buffer diluted urea solutions. Applications for the analysis of real samples are planned to do in the future.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalBiosensors and Bioelectronics
Volume18
Issue number2-3
DOIs
Publication statusPublished - Mar 1 2002

Fingerprint

Biosensing Techniques
Biosensors
Urea
Reaction Time
Light
Ions
Glass
Membranes
Enzymes
Ion selective membranes
Ionophores
Gas Lasers
Urease
Lasers
Spin coating
Photodetectors
Photodiodes
Transducers
Ammonium Compounds
Light sources

Keywords

  • Biosensor
  • Flow injection analysis
  • Planar wave-guide
  • Urea sensor

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Optical biosensor for urea with improved response time. / Kovács, Barna; Nagy, G.; Dombi, Roland; Tóth, K.

In: Biosensors and Bioelectronics, Vol. 18, No. 2-3, 01.03.2002, p. 111-118.

Research output: Contribution to journalArticle

Kovács, Barna ; Nagy, G. ; Dombi, Roland ; Tóth, K. / Optical biosensor for urea with improved response time. In: Biosensors and Bioelectronics. 2002 ; Vol. 18, No. 2-3. pp. 111-118.
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