Optical and structural properties of protein/gold hybrid bio-nanofilms prepared by layer-by-layer method

Edit Pál, Viktória Hornok, Dániel Sebk, Andrea Majzik, Imre Dékány

Research output: Contribution to journalArticle

10 Citations (Scopus)


Lysozyme/gold thin layers were prepared by layer-by-layer (LbL) self-assembly method. The build-up of the films was followed by UV-vis-absorbance spectra, quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) techniques. The structural property of films was examined by X-ray diffraction (XRD) measurements, while their morphology was studied by scanning electron microscopy (SEM) and atomic force microscopy (AFM). It was found that gold nanoparticles (NPs) had cubic crystalline structure, the primary particles form aggregates in the thin layer due to the presence of lysozyme molecules. The UV-vis measurements prove change in particle size while the colour of the film changes from wine-red to blue. The layer thickness of films was determined using the above methods and the loose, porous structure of the films explains the difference in the results. The vapour adsorption property of hybrid layers was also studied by QCM using different saturated vapours and ammonia gas. The lysozyme/Au films were most sensitive for ammonia gas among the tested gases/vapours due to the strongest interaction between the functional groups of the protein.

Original languageEnglish
Pages (from-to)276-283
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Issue number1
Publication statusPublished - Aug 1 2010


  • Gold, Lysozyme
  • Layer-by-layer
  • QCM
  • SPR
  • Thin films

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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