Opsin-immunoreactive outer segments of photoreceptors in the eye and in the lumen of the optic nerve of the hagfish, Myxine glutinosa

I. Vigh-Teichmann, B. Vigh, R. Olsson, Th van Veen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Opsin-immunoreactive sites in the eye and optic nerve of the hagfish, Myxine glutinosa, were studied by use of light-microscopic pre- and postembedding peroxidase-antiperoxidase or avidin-biotin-peroxidase techniques, and the immuno-electron-microscopic protein A-gold method. At the light-microscopic level, a strong opsin immuno-reaction was obtained on the outer segments of the photoreceptor cells with sheep and rat antibodies against bovine (rhod)opsin. These outer segments were located in the marginal photoreceptor space and in follicles of the retina, as well as in the tubular lumen of the optic nerve. Ultrastructurally, two classes of outer segments can be distinguished; most of them exhibited a strong antiopsin reaction, while certain elements lacked immunoreactivity with the antisera employed. The protein A-gold particles marked opsin-immunoreactive sites on the photoreceptor membranes. The presence of opsin-immunoreactive material in the retina and optic nerve of the hagfish strengthens the view that this primitive eye lacking a cornea, lens and vitreous body is engaged in light perception. The morphological similarity between the eye and pineal tissue is discussed in connection with the absence of a pineal organ in this species.

Original languageEnglish
Pages (from-to)515-522
Number of pages8
JournalCell And Tissue Research
Volume238
Issue number3
DOIs
Publication statusPublished - Nov 1 1984

    Fingerprint

Keywords

  • Cyclostome, Myxine glutinosa
  • Immunocytochemistry
  • Opsin
  • Optic nerve
  • Retinal photoreceptors

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this