Our aim was to investigate the role of adrenoceptors in the modulation of in vivo interleukin-10 (IL-10) production in lipopolysaccharide (LPS)-treated mice. The effect of different adrenergic drugs on plasma concentration of IL-10 was measured by ELISA 90 min after LPS injection. Our results confirmed the involvement of β-adrenoceptors since the β-agonist isoproterenol significantly increased the IL-10 production in response to LPS stimulation, whereas the β-antagonists propranolol decreased it. In contrast, the α2-agonists UK-14304, clonidine and xylazine significantly decreased the IL-10 plasma level, whereas the α2-antagonists CH-38083, prazosine and WB-4101 increased it. Our results provide the first in vivo evidence that, in addition to β-adrenoceptors, α2-adrenoceptors play also a very important role in the regulation of IL-10 production under endotoxaemic conditions. (C) 2000 Lippincott Williams and Wilkins.
- Neuroimmune communication
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