Opposite effects of molluscan neuropeptides CARP and MIP on LVA Ca-currents in Helix pomatia L. neurons

T. Kiss, Oleg N. Osipenko

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

1. Voltage-clamp recordings from identified neurons of Helix pomatia L. revealed that two Ca-current components may contribute to the inward current in Na-free saline. 2. It was found that the transient component of the inward Ca-current was largely blocked by Ni2+ but not by Cd2+. 3. CARP (10-6 M) selectively suppressed LVA currents. This effect is also reflected by tail current measurements, where in control saline the deactivation could be fitted by two exponentials, while after CARP treatment, by one. 4. MIP (10-6 M) specifically enhanced the LVA component, or shifted the I-V curve of the composite Ca-current in hyperpolarizing direction. 5. It is concluded that peptides (CARP and MIP) isolated from Mytilus ganglia selectively affect the LVA Ca-channels, and that this effect is supposed to be species-specific.

Original languageEnglish
Pages (from-to)21-27
Number of pages7
JournalComparative Biochemistry and Physiology. Part C, Comparative
Volume99
Issue number1-2
DOIs
Publication statusPublished - 1991

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Mytilus
Neuropeptides
Ganglia
Tail
Neurons
Peptides
Direction compound

ASJC Scopus subject areas

  • Pharmacology
  • Immunology

Cite this

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abstract = "1. Voltage-clamp recordings from identified neurons of Helix pomatia L. revealed that two Ca-current components may contribute to the inward current in Na-free saline. 2. It was found that the transient component of the inward Ca-current was largely blocked by Ni2+ but not by Cd2+. 3. CARP (10-6 M) selectively suppressed LVA currents. This effect is also reflected by tail current measurements, where in control saline the deactivation could be fitted by two exponentials, while after CARP treatment, by one. 4. MIP (10-6 M) specifically enhanced the LVA component, or shifted the I-V curve of the composite Ca-current in hyperpolarizing direction. 5. It is concluded that peptides (CARP and MIP) isolated from Mytilus ganglia selectively affect the LVA Ca-channels, and that this effect is supposed to be species-specific.",
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T1 - Opposite effects of molluscan neuropeptides CARP and MIP on LVA Ca-currents in Helix pomatia L. neurons

AU - Kiss, T.

AU - Osipenko, Oleg N.

PY - 1991

Y1 - 1991

N2 - 1. Voltage-clamp recordings from identified neurons of Helix pomatia L. revealed that two Ca-current components may contribute to the inward current in Na-free saline. 2. It was found that the transient component of the inward Ca-current was largely blocked by Ni2+ but not by Cd2+. 3. CARP (10-6 M) selectively suppressed LVA currents. This effect is also reflected by tail current measurements, where in control saline the deactivation could be fitted by two exponentials, while after CARP treatment, by one. 4. MIP (10-6 M) specifically enhanced the LVA component, or shifted the I-V curve of the composite Ca-current in hyperpolarizing direction. 5. It is concluded that peptides (CARP and MIP) isolated from Mytilus ganglia selectively affect the LVA Ca-channels, and that this effect is supposed to be species-specific.

AB - 1. Voltage-clamp recordings from identified neurons of Helix pomatia L. revealed that two Ca-current components may contribute to the inward current in Na-free saline. 2. It was found that the transient component of the inward Ca-current was largely blocked by Ni2+ but not by Cd2+. 3. CARP (10-6 M) selectively suppressed LVA currents. This effect is also reflected by tail current measurements, where in control saline the deactivation could be fitted by two exponentials, while after CARP treatment, by one. 4. MIP (10-6 M) specifically enhanced the LVA component, or shifted the I-V curve of the composite Ca-current in hyperpolarizing direction. 5. It is concluded that peptides (CARP and MIP) isolated from Mytilus ganglia selectively affect the LVA Ca-channels, and that this effect is supposed to be species-specific.

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