Abstract
The effect of cold-stress (2°C, 7 days cold-hardening) on the level of polyamines in different varieties of wheat and genetically modified wheat samples (chromosome substitution lines, deletion lines, recombinant lines) has been investigated. Biogenic amines (spermine, spermidine, putrescine, and agmatine) were determined as the dansyl derivatives by stepwise gradient elution with the Personal OPLC BS 50 Chromatograph. Short-term cold-hardening caused characteristic changes in free polyamine content of wheat genotypes. The total free polyamine content was significantly lower in the cold-hardened samples than in the control samples. It seems that accumulation of spermine and agmatine might be good markers of cold sensitivity.
| Original language | English |
|---|---|
| Pages (from-to) | 326-329 |
| Number of pages | 4 |
| Journal | Journal of Planar Chromatography - Modern TLC |
| Volume | 14 |
| Issue number | 5 |
| Publication status | Published - 2001 |
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Keywords
- Biogenic amines
- Cold stress
- OPLC
- Wheat
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
OPLC investigation of the effect of cold-hardening on the level of polyamines in wheat. / Simon-Sarkadi, L.; Kocsy, G.; Csomós, E.; Jakab, T.; Végh, Z.
In: Journal of Planar Chromatography - Modern TLC, Vol. 14, No. 5, 2001, p. 326-329.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - OPLC investigation of the effect of cold-hardening on the level of polyamines in wheat
AU - Simon-Sarkadi, L.
AU - Kocsy, G.
AU - Csomós, E.
AU - Jakab, T.
AU - Végh, Z.
PY - 2001
Y1 - 2001
N2 - The effect of cold-stress (2°C, 7 days cold-hardening) on the level of polyamines in different varieties of wheat and genetically modified wheat samples (chromosome substitution lines, deletion lines, recombinant lines) has been investigated. Biogenic amines (spermine, spermidine, putrescine, and agmatine) were determined as the dansyl derivatives by stepwise gradient elution with the Personal OPLC BS 50 Chromatograph. Short-term cold-hardening caused characteristic changes in free polyamine content of wheat genotypes. The total free polyamine content was significantly lower in the cold-hardened samples than in the control samples. It seems that accumulation of spermine and agmatine might be good markers of cold sensitivity.
AB - The effect of cold-stress (2°C, 7 days cold-hardening) on the level of polyamines in different varieties of wheat and genetically modified wheat samples (chromosome substitution lines, deletion lines, recombinant lines) has been investigated. Biogenic amines (spermine, spermidine, putrescine, and agmatine) were determined as the dansyl derivatives by stepwise gradient elution with the Personal OPLC BS 50 Chromatograph. Short-term cold-hardening caused characteristic changes in free polyamine content of wheat genotypes. The total free polyamine content was significantly lower in the cold-hardened samples than in the control samples. It seems that accumulation of spermine and agmatine might be good markers of cold sensitivity.
KW - Biogenic amines
KW - Cold stress
KW - OPLC
KW - Wheat
UR - http://www.scopus.com/inward/record.url?scp=0035657078&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035657078&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0035657078
VL - 14
SP - 326
EP - 329
JO - Journal of Planar Chromatography - Modern TLC
JF - Journal of Planar Chromatography - Modern TLC
SN - 0933-4173
IS - 5
ER -