OPLC investigation of the effect of cold-hardening on the level of polyamines in wheat

L. Simon-Sarkadi, G. Kocsy, E. Csomós, T. Jakab, Z. Végh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effect of cold-stress (2°C, 7 days cold-hardening) on the level of polyamines in different varieties of wheat and genetically modified wheat samples (chromosome substitution lines, deletion lines, recombinant lines) has been investigated. Biogenic amines (spermine, spermidine, putrescine, and agmatine) were determined as the dansyl derivatives by stepwise gradient elution with the Personal OPLC BS 50 Chromatograph. Short-term cold-hardening caused characteristic changes in free polyamine content of wheat genotypes. The total free polyamine content was significantly lower in the cold-hardened samples than in the control samples. It seems that accumulation of spermine and agmatine might be good markers of cold sensitivity.

Original languageEnglish
Pages (from-to)326-329
Number of pages4
JournalJournal of Planar Chromatography - Modern TLC
Volume14
Issue number5
Publication statusPublished - Dec 1 2001

Keywords

  • Biogenic amines
  • Cold stress
  • OPLC
  • Wheat

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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