Opioid peptides in the nervous system of Aplysia: A combined biochemical, immunocytochemical, and electrophysiological study

D. O. Carpenter, G. Kemenes, K. Elekes, M. Leung, G. Stefano, K. S.-Rózsa, J. Salánki

Research output: Contribution to journalArticle

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Abstract

1. We have used biochemical, immunocytochemical, and electrophysiological techniques to evaluate the role of opioid peptides in the central nervous system of the marine mollusc, Aplysia california. 2. Binding studies using3H-d-Ala2, met-enkephalinamide (3H-DAMA) showed a single class of high-affinity binding sites with a Kd of 1.3 n M and a binding density of 45 pmol/g. 3. HPLC extracts of ganglia revealed multiple peaks with immunoreactivity for either leu (LEU-IR)- or met-enkephalin (MET-IR), but the amounts were not uniformly distributed in all ganglia. 4. LEU-IR and MET-IR neurons were demonstrated immunocytochemically in all ganglia, but MET-IR neurons were more frequent and were concentrated in pedal and pleural ganglia. While absorption control studies abolished MET-IR, LEU-IR was only partially abolished in the neuropil. 5. In electrophysiological studies, both depolarizing and hyperpolarizing responses were found to d-Ala2-leu-enkephalin (DALEU) and d-Ala2-met enkephalin (DAMET) on some and different neurons. 6. HPLC fractions from regions with retention times corresponding to authentic leu- or met-enkephalin showed physiologic responses similar to those of DALEU and DAMET, respectively. 7. These studies suggest that a variety of endogeneous opioid peptides play physiologically important roles in the nervous system of Aplysia, including but not necessarily limited to leu- and met-enkephalin.

Original languageEnglish
Pages (from-to)239-256
Number of pages18
JournalCellular and Molecular Neurobiology
Volume15
Issue number2
DOIs
Publication statusPublished - Apr 1995

Fingerprint

Aplysia
Methionine Enkephalin
Opioid Peptides
Neurology
Leucine Enkephalin
Nervous System
Ganglia
Neurons
High Pressure Liquid Chromatography
Molluscs
Neuropil
Mollusca
Foot
Central Nervous System
Binding Sites

Keywords

  • Aplysia
  • binding sites
  • electrophysiology
  • immunocytochemistry
  • leu-enkephalin
  • met-enkephalin
  • opioid receptors

ASJC Scopus subject areas

  • Neuroscience(all)
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

Cite this

Opioid peptides in the nervous system of Aplysia : A combined biochemical, immunocytochemical, and electrophysiological study. / Carpenter, D. O.; Kemenes, G.; Elekes, K.; Leung, M.; Stefano, G.; S.-Rózsa, K.; Salánki, J.

In: Cellular and Molecular Neurobiology, Vol. 15, No. 2, 04.1995, p. 239-256.

Research output: Contribution to journalArticle

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