Opines in naturally infected grapevine crown gall tumors

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15 Citations (Scopus)


Crown gall tumors collected from naturally infected plants of 7 grapevine varieties were analysed for the presence of opines. Eighty-five of the tested 90 samples contained known Agrobacterium vitis-induced opines. Octopine was the most common, it was found in 50 samples. Twenty-eight crown galls contained nopaline and 8 were vitopine positive. There was only one tumor that contained two opines, nopaline and vitopine. Five samples were negative for the opines tested. The presence of A. vitis was confirmed in most of these tumors by PCR analysis. The opine assay may provide a simple diagnostic protocol to distinguish healthy callus tissues from crown galls as well as for the indirect identification of Agrobacterium infection.

Original languageEnglish
Pages (from-to)39-41
Number of pages3
Issue number1
Publication statusPublished - Apr 16 2003


  • Agrobacterium vitis
  • Nopaline
  • Octopine
  • Polymerase chain reaction
  • Vitis vinifera
  • Vitopine

ASJC Scopus subject areas

  • Genetics
  • Horticulture

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