Ophiobolin A from Bipolaris oryzae perturbs motility and membrane integrities of porcine sperm and induces cell death on mammalian somatic cell lines

Ottó Bencsik, T. Papp, Máté Berta, Annamária Zana, P. Forgó, G. Dombi, Maria A. Andersson, Mirja Salkinoja-Salonen, C. Vágvölgyi, A. Szekeres

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Bipolaris oryzae is a phytopathogenic fungus causing a brown spot disease in rice, and produces substance that strongly perturbs motility and membrane integrities of boar spermatozoa. The substance was isolated from the liquid culture of the fungal strain using extraction and a multi-step semi-preparative HPLC procedures. Based on the results of mass spectrometric and 2D NMR techniques, the bioactive molecule was identified as ophiobolin A, a previously described sesterterpene-type compound. The purified ophiobolin A exhibited strong motility inhibition and viability reduction on boar spermatozoa. Furthermore, it damaged the sperm mitochondria significantly at sublethal concentration by the dissipation of transmembrane potential in the mitochondrial inner membrane, while the plasma membrane permeability barrier remained intact. The study demonstrated that the cytotoxicity of ophiobolin A toward somatic cell lines is higher by 1-2 orders of magnitude compared to other mitochondriotoxic mycotoxins, and towards sperm cells unique by replacing the progressive motility by shivering tail beating at low exposure concentration.

Original languageEnglish
Pages (from-to)2857-2871
Number of pages15
JournalToxins
Volume6
Issue number9
DOIs
Publication statusPublished - Sep 1 2014

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Cell death
Spermatozoa
Cell Death
Swine
Cells
Membranes
Cell Line
Sesterterpenes
Mitochondria
Mycotoxins
Cell membranes
Cytotoxicity
Fungi
Shivering
Mitochondrial Membranes
Nuclear magnetic resonance
Membrane Potentials
Permeability
Molecules
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ophiobolin A from Bipolaris oryzae perturbs motility and membrane integrities of porcine sperm and induces cell death on mammalian somatic cell lines. / Bencsik, Ottó; Papp, T.; Berta, Máté; Zana, Annamária; Forgó, P.; Dombi, G.; Andersson, Maria A.; Salkinoja-Salonen, Mirja; Vágvölgyi, C.; Szekeres, A.

In: Toxins, Vol. 6, No. 9, 01.09.2014, p. 2857-2871.

Research output: Contribution to journalArticle

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