Operon flv4-flv2 provides cyanobacterial photosystem ii with flexibility of electron transfer

Pengpeng Zhang, Marion Eisenhut, Anna Maria Brandt, Dalton Carmel, Henna M. Silén, I. Vass, Yagut Allahverdiyeva, Tiina A. Salminen, Eva Mari Aro

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Synechocystis sp PCC 6803 has four genes encoding flavodiiron proteins (FDPs; Flv1 to Flv4). Here, we investigated the flv4- flv2 operon encoding the Flv4, Sll0218, and Flv2 proteins, which are strongly expressed under low inorganic carbon conditions (i.e., air level of CO2) but become repressed at elevated CO2 conditions. Different from FDP homodimers in anaerobic microbes, Synechocystis Flv2 and Flv4 form a heterodimer. It is located in cytoplasm but also has a high affinity to membrane in the presence of cations. Sll0218, on the contrary, resides in the thylakoid membrane in association with a high molecular mass protein complex. Sll0218 operates partially independently of Flv2/Flv4. It stabilizes the photosystem II (PSII) dimers, and according to biophysical measurements opens up a novel electron transfer pathway to the Flv2/Flv4 heterodimer from PSII. Constructed homology models suggest efficient electron transfer in heterodimeric Flv2/Flv4. It is suggested that Flv2/Flv4 binds to thylakoids in light, mediates electron transfer from PSII, and concomitantly regulates the association of phycobilisomes with PSII. The function of the flv4-flv2 operon provides many β-cyanobacteria with a so far unknown photoprotection mechanism that evolved in parallel with oxygen-evolving PSII.

Original languageEnglish
Pages (from-to)1952-1971
Number of pages20
JournalPlant Cell
Volume24
Issue number5
DOIs
Publication statusPublished - May 2012

Fingerprint

Photosystem II Protein Complex
operon
Operon
photosystem II
electron transfer
Electrons
Synechocystis
Thylakoids
thylakoids
Phycobilisomes
carbon dioxide
phycobilisome
Proteins
proteins
photostability
Cyanobacteria
Cations
cations
Cytoplasm
Carbon

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Zhang, P., Eisenhut, M., Brandt, A. M., Carmel, D., Silén, H. M., Vass, I., ... Aro, E. M. (2012). Operon flv4-flv2 provides cyanobacterial photosystem ii with flexibility of electron transfer. Plant Cell, 24(5), 1952-1971. https://doi.org/10.1105/tpc.111.094417

Operon flv4-flv2 provides cyanobacterial photosystem ii with flexibility of electron transfer. / Zhang, Pengpeng; Eisenhut, Marion; Brandt, Anna Maria; Carmel, Dalton; Silén, Henna M.; Vass, I.; Allahverdiyeva, Yagut; Salminen, Tiina A.; Aro, Eva Mari.

In: Plant Cell, Vol. 24, No. 5, 05.2012, p. 1952-1971.

Research output: Contribution to journalArticle

Zhang, P, Eisenhut, M, Brandt, AM, Carmel, D, Silén, HM, Vass, I, Allahverdiyeva, Y, Salminen, TA & Aro, EM 2012, 'Operon flv4-flv2 provides cyanobacterial photosystem ii with flexibility of electron transfer', Plant Cell, vol. 24, no. 5, pp. 1952-1971. https://doi.org/10.1105/tpc.111.094417
Zhang, Pengpeng ; Eisenhut, Marion ; Brandt, Anna Maria ; Carmel, Dalton ; Silén, Henna M. ; Vass, I. ; Allahverdiyeva, Yagut ; Salminen, Tiina A. ; Aro, Eva Mari. / Operon flv4-flv2 provides cyanobacterial photosystem ii with flexibility of electron transfer. In: Plant Cell. 2012 ; Vol. 24, No. 5. pp. 1952-1971.
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