Operating conditions in preparative medium pressure liquid chromatography (MPLC).I. Influence of column preparation and particle size of silica

G. C. Zogg, SZ Nyiredy, O. Sticher

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Various methods were tested for packing columns with silica having an average particle size of 15 μm for use in preparative medium pressure liquid chromatography (MPLC). Depending on the column type investigated, of the five methods tested, the two newly elaborated dry-packing procedures, using vacuum and nitrogen overpressure, resulted in the best packing density. Using these dry-filling techniques, the influence of various types of silica [with different average particle sizes (15 μm up to 130 μm) and particle size distributions (5-25 μm up to 63-200 μm)] on resolution and retention times was examined. Evaluation with different particle sizes showed a pronounced decrease of band broadening on using silica with an average particle size of 15 μm, which in turn resulted in a significant increase of resolution. The fluorescence of the test compounds used permitted observation of the chromatographic development. For preparative MPLC separations of the closely related test compounds a significant increase in resolution was obtained using silica with small particles and a narrow particle size distribution.

Original languageEnglish
Pages (from-to)2031-2048
Number of pages18
JournalJournal of Liquid Chromatography
Volume12
Issue number11
Publication statusPublished - Sep 1 1989

ASJC Scopus subject areas

  • Molecular Medicine

Fingerprint Dive into the research topics of 'Operating conditions in preparative medium pressure liquid chromatography (MPLC).I. Influence of column preparation and particle size of silica'. Together they form a unique fingerprint.

  • Cite this