Oocyte cryopreservation

The birth of the first Hungarian babies from frozen oocytes

Janos Konc, Katalin Kanyo, Erika Varga, Rita Kriston, S. Cseh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose: To present data obtained with clinical application of oocyte cryopreservation. Methods: Slow freezing/rapid thawing in PBS based medium containing 1.5 M propanediol + 0.3 M sucrose. Results: A total of 127 embryos were transferred into 54 patients (1.9 embryo/cycle, 64 transfer cycles). Clinical pregnancy rate of 20% per cycle (13/64) and 24.0% per patient were achieved. Up-to-date, six patients delivered seven healthy babies; there are four ongoing pregnancies. Three abortions (23%) and one biochemical pregnancy (0.7%) was obtained. Implantation rates of 11% per transferred embryos (14/127) and 6.5% (14/215) per thawed eggs were found. In each case, normal karyotype was detected. No difference was found in the ratio of spindle positive oocytes at the polscope analyses done before and after freezing (75.8% vs. 82.5%). Conclusion: Egg freezing is not a routine procedure yet, but there will certainly be a place for it in the future of assisted reproductive medicine.

Original languageEnglish
Pages (from-to)349-352
Number of pages4
JournalJournal of Assisted Reproduction and Genetics
Volume25
Issue number7
DOIs
Publication statusPublished - Jul 2008

Fingerprint

Birth Order
Cryopreservation
Freezing
Oocytes
Embryonic Structures
Propylene Glycols
Reproductive Medicine
Pregnancy
Pregnancy Rate
Karyotype
Eggs
Ovum
Sucrose

Keywords

  • Oocyte cryopreservation
  • Oocyte survival
  • Propanediol
  • Slow freezing
  • Sucrose

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics
  • Reproductive Medicine

Cite this

Oocyte cryopreservation : The birth of the first Hungarian babies from frozen oocytes. / Konc, Janos; Kanyo, Katalin; Varga, Erika; Kriston, Rita; Cseh, S.

In: Journal of Assisted Reproduction and Genetics, Vol. 25, No. 7, 07.2008, p. 349-352.

Research output: Contribution to journalArticle

Konc, Janos ; Kanyo, Katalin ; Varga, Erika ; Kriston, Rita ; Cseh, S. / Oocyte cryopreservation : The birth of the first Hungarian babies from frozen oocytes. In: Journal of Assisted Reproduction and Genetics. 2008 ; Vol. 25, No. 7. pp. 349-352.
@article{42a05726630144929d54b3776684d49a,
title = "Oocyte cryopreservation: The birth of the first Hungarian babies from frozen oocytes",
abstract = "Purpose: To present data obtained with clinical application of oocyte cryopreservation. Methods: Slow freezing/rapid thawing in PBS based medium containing 1.5 M propanediol + 0.3 M sucrose. Results: A total of 127 embryos were transferred into 54 patients (1.9 embryo/cycle, 64 transfer cycles). Clinical pregnancy rate of 20{\%} per cycle (13/64) and 24.0{\%} per patient were achieved. Up-to-date, six patients delivered seven healthy babies; there are four ongoing pregnancies. Three abortions (23{\%}) and one biochemical pregnancy (0.7{\%}) was obtained. Implantation rates of 11{\%} per transferred embryos (14/127) and 6.5{\%} (14/215) per thawed eggs were found. In each case, normal karyotype was detected. No difference was found in the ratio of spindle positive oocytes at the polscope analyses done before and after freezing (75.8{\%} vs. 82.5{\%}). Conclusion: Egg freezing is not a routine procedure yet, but there will certainly be a place for it in the future of assisted reproductive medicine.",
keywords = "Oocyte cryopreservation, Oocyte survival, Propanediol, Slow freezing, Sucrose",
author = "Janos Konc and Katalin Kanyo and Erika Varga and Rita Kriston and S. Cseh",
year = "2008",
month = "7",
doi = "10.1007/s10815-008-9235-0",
language = "English",
volume = "25",
pages = "349--352",
journal = "Journal of Assisted Reproduction and Genetics",
issn = "1058-0468",
publisher = "Springer New York",
number = "7",

}

TY - JOUR

T1 - Oocyte cryopreservation

T2 - The birth of the first Hungarian babies from frozen oocytes

AU - Konc, Janos

AU - Kanyo, Katalin

AU - Varga, Erika

AU - Kriston, Rita

AU - Cseh, S.

PY - 2008/7

Y1 - 2008/7

N2 - Purpose: To present data obtained with clinical application of oocyte cryopreservation. Methods: Slow freezing/rapid thawing in PBS based medium containing 1.5 M propanediol + 0.3 M sucrose. Results: A total of 127 embryos were transferred into 54 patients (1.9 embryo/cycle, 64 transfer cycles). Clinical pregnancy rate of 20% per cycle (13/64) and 24.0% per patient were achieved. Up-to-date, six patients delivered seven healthy babies; there are four ongoing pregnancies. Three abortions (23%) and one biochemical pregnancy (0.7%) was obtained. Implantation rates of 11% per transferred embryos (14/127) and 6.5% (14/215) per thawed eggs were found. In each case, normal karyotype was detected. No difference was found in the ratio of spindle positive oocytes at the polscope analyses done before and after freezing (75.8% vs. 82.5%). Conclusion: Egg freezing is not a routine procedure yet, but there will certainly be a place for it in the future of assisted reproductive medicine.

AB - Purpose: To present data obtained with clinical application of oocyte cryopreservation. Methods: Slow freezing/rapid thawing in PBS based medium containing 1.5 M propanediol + 0.3 M sucrose. Results: A total of 127 embryos were transferred into 54 patients (1.9 embryo/cycle, 64 transfer cycles). Clinical pregnancy rate of 20% per cycle (13/64) and 24.0% per patient were achieved. Up-to-date, six patients delivered seven healthy babies; there are four ongoing pregnancies. Three abortions (23%) and one biochemical pregnancy (0.7%) was obtained. Implantation rates of 11% per transferred embryos (14/127) and 6.5% (14/215) per thawed eggs were found. In each case, normal karyotype was detected. No difference was found in the ratio of spindle positive oocytes at the polscope analyses done before and after freezing (75.8% vs. 82.5%). Conclusion: Egg freezing is not a routine procedure yet, but there will certainly be a place for it in the future of assisted reproductive medicine.

KW - Oocyte cryopreservation

KW - Oocyte survival

KW - Propanediol

KW - Slow freezing

KW - Sucrose

UR - http://www.scopus.com/inward/record.url?scp=50249147724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50249147724&partnerID=8YFLogxK

U2 - 10.1007/s10815-008-9235-0

DO - 10.1007/s10815-008-9235-0

M3 - Article

VL - 25

SP - 349

EP - 352

JO - Journal of Assisted Reproduction and Genetics

JF - Journal of Assisted Reproduction and Genetics

SN - 1058-0468

IS - 7

ER -