Ontogeny of angiotensin II type 1 receptor mRNAs in fetal and neonatal rat brain

Anne Monique Nuyt, Zsolt Lenkei, Pierre Corvol, M. Palkóvits, Catherine Llorens-Corts

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Studies have demonstrated a specific function of the angiotensin II (Ang II) type 1 receptor (AT1) in regulation of adult central cardiovascular, fluid, and pituitary hormone release and a predominant role of the renin-angiotensin system in fetal and neonatal cardiovascular homeostasis. The pattern of brain AT1 mRNA expression during fetal and neonatal development is currently unknown. We used radiolabeled cRNA probes for in situ hybridization histochemistry to determine the ontogenic development of the two AT1 subtypes (AT1a and AT1b) mRNA in rat brain, from 11 days of gestation (E11) to 28 days after birth (P28). No AT1b mRNA was detected in the developing brain, whereas AT1a mRNA was first detected at E19. The age at which AT1a mRNA is first detected varied among different brain areas and expression predominates in areas involved in fluid homeostasis, pituitary hormone release, and cardiovascular regulation, where it persists until P28. AT1a mRNA expression is present from E19 onward in the median preoptic nucleus, the vascular organ of the lamina terminalis, the paraventricular nucleus, the periaqueductal gray, the nucleus raphe pallidus, the motor facial nucleus, and very weakly in the nucleus of the solitary tract and the ambiguus nucleus, and at E21 in the subfornical organ, the anterior olfactory nucleus and the piriform cortex. AT1a mRNA expression is present after birth in many regions, including the preoptic and lateral hypothalamic areas, the area postrema and medullary reticular nuclei. In conclusion, during brain development, expression of AT1a mRNA, appears in late gestation at E19, predominantly in forebrain areas involved in fluid homeostasis and cardiovascular regulation. In contrast, AT1a mRNA expression is absent or present only in very small amounts until after birth in many medullary nuclei, known to play an important role in cardiovascular modulation. Our results suggest that, in perinatal life, AT1a is involved in fluid and perhaps cardiovascular homeostasis and that the role of Ang II in modulating medullary cardiovascular centers matures later in postnatal life.

Original languageEnglish
Pages (from-to)192-203
Number of pages12
JournalJournal of Comparative Neurology
Volume440
Issue number2
DOIs
Publication statusPublished - Nov 12 2001

Fingerprint

Angiotensin Type 1 Receptor
Messenger RNA
Brain
Homeostasis
Solitary Nucleus
Pituitary Hormones
Parturition
Subfornical Organ
Lateral Hypothalamic Area
Area Postrema
Pregnancy
Complementary RNA
Periaqueductal Gray
Preoptic Area
Paraventricular Hypothalamic Nucleus
Renin-Angiotensin System
Prosencephalon
Fetal Development
Angiotensin II
Hypothalamus

Keywords

  • Brain mapping
  • Fetus
  • In situ hybridization
  • Newborn
  • Receptor development

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ontogeny of angiotensin II type 1 receptor mRNAs in fetal and neonatal rat brain. / Nuyt, Anne Monique; Lenkei, Zsolt; Corvol, Pierre; Palkóvits, M.; Llorens-Corts, Catherine.

In: Journal of Comparative Neurology, Vol. 440, No. 2, 12.11.2001, p. 192-203.

Research output: Contribution to journalArticle

Nuyt, Anne Monique ; Lenkei, Zsolt ; Corvol, Pierre ; Palkóvits, M. ; Llorens-Corts, Catherine. / Ontogeny of angiotensin II type 1 receptor mRNAs in fetal and neonatal rat brain. In: Journal of Comparative Neurology. 2001 ; Vol. 440, No. 2. pp. 192-203.
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