Onset of carbon catabolite repression in Aspergillus nidulans: Parallel involvement of hexokinase and glucokinase in sugar signaling

M. Flipphi, Peter J I Van de Vondervoort, George J G Ruijter, Jaap Visser, Herbert N. Arst, Béatrice Felenbok

Research output: Contribution to journalArticle

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Abstract

The role of hexose phosphorylating enzymes in the signaling of carbon catabolite repression was investigated in the filamentous fungus Aspergillus nidulans. A D-fructose non-utilizing, hexokinase-deficient (hxkA1, formerly designated frA1) strain was utilized to obtain new mutants lacking either glucokinase (glkA4) or both hexose kinases (hxkA1/glkA4). D-Glucose and D-fructose phosphorylation is completely abolished in the double mutant, which consequently cannot grow on either sugar. The glucokinase single mutant exhibits no nutritional deficiencies. Three repressible diagnostic systems, ethanol utilization (alcA and alcR genes), xylan degradation (xlnA), and acetate catabolism (facA), were analyzed in these hexose kinase mutants at the transcript level. Transcriptional repression by D-glucose is fully retained in the two single kinase mutants, whereas the hexokinase mutant is partially derepressed for D-fructose. Thus, hexokinase A and glucokinase A compensate each other for carbon catabolite repression by D-glucose in the single mutants. In contrast, both D-glucose and D-fructose repression are severely impaired for all three diagnostic systems in the double mutant. Unlike the situation in Saccharomyces cerevisiae, the hexose phosphorylating enzymes play parallel roles in glucose repression in A. nidulans.

Original languageEnglish
Pages (from-to)11849-11857
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number14
DOIs
Publication statusPublished - Apr 4 2003

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Catabolite Repression
Glucokinase
Aspergillus nidulans
Hexokinase
Aspergillus
Sugars
Hexoses
Fructose
Carbon
Glucose
Phosphotransferases
Xylans
Phosphorylation
Enzymes
Fungi
Malnutrition
Yeast
Saccharomyces cerevisiae
Acetates
Ethanol

ASJC Scopus subject areas

  • Biochemistry

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Onset of carbon catabolite repression in Aspergillus nidulans : Parallel involvement of hexokinase and glucokinase in sugar signaling. / Flipphi, M.; Van de Vondervoort, Peter J I; Ruijter, George J G; Visser, Jaap; Arst, Herbert N.; Felenbok, Béatrice.

In: Journal of Biological Chemistry, Vol. 278, No. 14, 04.04.2003, p. 11849-11857.

Research output: Contribution to journalArticle

Flipphi, M. ; Van de Vondervoort, Peter J I ; Ruijter, George J G ; Visser, Jaap ; Arst, Herbert N. ; Felenbok, Béatrice. / Onset of carbon catabolite repression in Aspergillus nidulans : Parallel involvement of hexokinase and glucokinase in sugar signaling. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 14. pp. 11849-11857.
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