On the substrate specificity of the red cell calcium pump

A. Enyedi, B. Sarkadi, G. Gárdos

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

ATP-dependent active calcium transport in inside-out human red cell membrane vesicles is stimulated by magnesium essentially parallel with an increase in MgATP concentration. At a constant, low (1 μM) calcium concentration, increasing ATP and magnesium increase the maximum calcium transport rate irrespective of the constant or decreasing concentrations of CaATP present. KCa for calcium pumping is practically unchanged at variable ATP and magnesium concentrations. Free magnesium above 1-2 mM inhibits active calcium transport, probably through a direct interaction with the transport enzyme. Based on the experimental findings reported we suggest that the true, physiological substrate of the red cell calcium pump is MgATP.

Original languageEnglish
Pages (from-to)109-112
Number of pages4
JournalBBA - Biomembranes
Volume687
Issue number1
DOIs
Publication statusPublished - Apr 23 1982

Fingerprint

Substrate Specificity
Adenosine Triphosphate
Cells
Pumps
Calcium
Magnesium
Substrates
Active Biological Transport
Cell membranes
Cell Membrane
Enzymes

Keywords

  • (Erythrocyte membrane)
  • Ca transport
  • Inside-out vesicle
  • MgATP
  • Substrate specificity

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Medicine(all)

Cite this

On the substrate specificity of the red cell calcium pump. / Enyedi, A.; Sarkadi, B.; Gárdos, G.

In: BBA - Biomembranes, Vol. 687, No. 1, 23.04.1982, p. 109-112.

Research output: Contribution to journalArticle

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