On the Mechanism of the Involvement of Monoamine Oxidase in Catecholamine‐Stimulated Prostaglandin Biosynthesis in Particulate Fraction of Rat Brain Homogenates: Role of Hydrogen Peroxide

András Seregi, Péter Serfözö, Zsuzsanna Mergl, András Schaefer

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Abstract: The mechanism of involvement of monoamine oxidase (MAO) in catecholamine‐stimulated prostaglandin (PG) biosynthesis was studied in the particulate fraction of rat brain homogenates. High concentrations of either noradrenaline (NA) or dopamine (DA) stimulated effectively PGF formation. The same amount of 2‐phenylethylamine (PEA) acted similarly, provided that it was administered together with a catecholamine analogue or metabolite possessing the 3,4‐dihydroxyphenyl nucleus–3, 4‐dihydroxyphenylalanine (DOPA), 3,4‐dihydroxyphenylacetic acid (DOPAC), 3,4‐dihydroxyphenyl‐glycol (DOPEG), 3,4‐dihydroxyphenylacetaldehyde (DOPAL), or α‐methylnoradrenaline (α‐met‐NA)–or with SnCl2. In the absence of PEA, these compounds were ineffective with regard to stimulation of PGF formation. Catalase, pargyline, or indomethacin abolished completely PGF formation elicited either by catecholamines or by PEA plus a 3,4‐dihydroxyphenyl compound or SnCl2. With regard to the stimulation of PGF formation in the presence of α‐met‐NA, PEA could be replaced by H2O2, generated by the glucose oxidase(GOD)‐glucose system. The effect of H2O2 was inhibited by indomethacin or catalase, but pargyline was ineffective. It is assumed that catecholamines play a dual role in the activation of PG biosynthesis in brain tissue. During the enzymatic decomposition of catecholamines MAO produces H2O2, which stimulates endoperoxide synthesis. Simultaneously, catecholamines as hydrogen donors promote the nonenzymatic transformation of endoperoxides into PGF. The possible physiological importance of these findings is discussed.

Original languageEnglish
Pages (from-to)20-27
Number of pages8
JournalJournal of neurochemistry
Volume38
Issue number1
DOIs
Publication statusPublished - Jan 1982

Keywords

  • Brain tissue
  • Catecholamines
  • Hydrogen peroxide
  • Monoamine oxidase
  • Prostaglandin biosynthesis

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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