Háromfázisú megoszlással készí tett fehérjegélek mechanikai tulajdonságai

Translated title of the contribution: On the elastic behaviour of protein gels prepared by interfacial partitioning

Réka Borbás, E. Kiss, Miklós Nagy

Research output: Contribution to journalArticle

Abstract

Treating an aqueous protein solution with tert-butanol and ammonium sulphate in a suitable proportion leads to the formation of a coherent protein-rich phase. This process is called three-phase partitioning, TPP. The gel-like phase is located in between two liquid phases, which are composed of tert-butanol, ammonium sulphate, water and protein. The mechanical characterisation of middle phase was carried out in model systems using bovine serum albumin or ovalbumin. Stress - deformation relationship obtained by uniaxial compression showed the elastic behaviour of the middle phase. It was found that the elastic modulus of the protein gel increased with stress. The middle phase became harder upon compression, possibly due to some change in structure. The deformation - time measurements provided information on the viscoelastic properties of the gels and on reversibility of deformation. The BSA gels showed higher elastic and viscous deformation and lower reversibility than ovalbumin gels. The rheological behaviour of protein gels is consistent with the previous results obtained from the analysis of middle phase components. On the basis of these facts, it is suggested that the middle phase formed in TPP is a concentrated emulsion, probably an emulsion gel.

Original languageHungarian
Pages (from-to)163-169
Number of pages7
JournalMagyar Kemiai Folyoirat, Kemiai Kozlemenyek
Volume108
Issue number4
Publication statusPublished - Apr 2002

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Gels
tert-Butyl Alcohol
Proteins
Ovalbumin
Ammonium Sulfate
Emulsions
Bovine Serum Albumin
Time measurement
Compaction
Elastic moduli
Water
Liquids

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Háromfázisú megoszlással készí tett fehérjegélek mechanikai tulajdonságai. / Borbás, Réka; Kiss, E.; Nagy, Miklós.

In: Magyar Kemiai Folyoirat, Kemiai Kozlemenyek, Vol. 108, No. 4, 04.2002, p. 163-169.

Research output: Contribution to journalArticle

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