On the background of enhanced stability and reusability of enzymes in ionic liquids

E. Fehér, B. Major, K. Bélafi-Bakó, L. Gubicza

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

The ability of ILs (ionic liquids) to provide an environment of increased stability and, in this way, improve the recyclability of enzymes has been studied. The description of this phenomenon is not easy; there are several approaches for explanation. In this mini-review, the results from different research groups are summarized, with the aim of explaining the strong stability effect of ILs on several enzymes. Spectroscopic methods (e.g. fluorescence and CD, IR spectroscopy, mass spectroscopy and NMR) and investigations of polarity and kosmotropicity of ions are promising methods. Since higher stability means that we may be able to reuse enzymes more times, the recyclability of enzymes was also in the focus. From this point of view, the advantages and disadvantages of applying monophasic or biphasic systems are discussed too, presenting the coupled techniques as well.

Original languageEnglish
Pages (from-to)1624-1627
Number of pages4
JournalBiochemical Society Transactions
Volume35
Issue number6
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Enzyme Stability
Ionic Liquids
Reusability
Enzymes
Infrared spectroscopy
Mass Spectrometry
Magnetic Resonance Spectroscopy
Fluorescence
Nuclear magnetic resonance
Spectroscopy
Ions
Research

Keywords

  • Biphasic reaction
  • Hydrogen-bond network
  • Ionic liquid
  • Kosmotropicity
  • Monophasic reaction
  • Recycling

ASJC Scopus subject areas

  • Biochemistry

Cite this

On the background of enhanced stability and reusability of enzymes in ionic liquids. / Fehér, E.; Major, B.; Bélafi-Bakó, K.; Gubicza, L.

In: Biochemical Society Transactions, Vol. 35, No. 6, 12.2007, p. 1624-1627.

Research output: Contribution to journalArticle

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