Isolated aquatic humic and fulvic acids were analysed with on- line size exclusion chromatography/electrospray ionisation mass spectrometry (SEC/ESI-MS). An eluent composition which enabled electrospray ionisation was identified. The SEC separation improved interpretability of mass spectra and may open up new possibilities for molecular weight determination of humic substances. A linear dose-response relationship over a factor of 20 was obtained and the limit of detection was 50ng/uL for humic and fulvic acids. Spectral changes due to different ionisation conditions (pH and cone voltage) were investigated. A natural water sample from a Swedish lake was analysed. Copyright (C) 2000 John Wiley and Sons, Ltd.
|Number of pages||7|
|Journal||Rapid Communications in Mass Spectrometry|
|Publication status||Published - Feb 28 2000|
ASJC Scopus subject areas
- Analytical Chemistry
- Organic Chemistry