On-line multidimensional open tubular column supercritical fluid chromatography using a valve-switching interface

L. Q. Xie, Z. Juvancz, K. E. Markides, M. L. Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

An on-line two-dimensional open tubular column supercritical fluid chromatograph was constructed and evaluated. A rotary valve interface allowed independent flow control of two 50-μm i.d. open tubular columns, providing maximum versatility for heartcutting. A solvent-venting injection technique was incorporated in the system that enabled single or multiple 2.0-μL volumes to be injected into an uncoated, yet deactivated, length of capillary precolumn without flooding of the analytical column. A cold trap was employed to refocussolutes from single or multiple fractional cuts after being transferred to the second dimension. The performance of the system was demonstrated with the analysis of mixtures of polycyclic aromatic compounds and steroids. Efficiencies of 4,500 plates m-1 were preserved in the second column after heartcutting.

Original languageEnglish
Pages (from-to)233-238
Number of pages6
JournalChromatographia
Volume31
Issue number5-6
DOIs
Publication statusPublished - Mar 1991

Fingerprint

Polycyclic Compounds
Supercritical Fluid Chromatography
Supercritical fluids
Aromatic compounds
Flow control
Steroids
Injections

Keywords

  • Multidimensional chromatography
  • Open tubular columns
  • Supercritical fluid chromatography
  • Valve switching

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

On-line multidimensional open tubular column supercritical fluid chromatography using a valve-switching interface. / Xie, L. Q.; Juvancz, Z.; Markides, K. E.; Lee, M. L.

In: Chromatographia, Vol. 31, No. 5-6, 03.1991, p. 233-238.

Research output: Contribution to journalArticle

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