On-column transient and coupled column isotachophoretic preconcentration of protein samples in capillary zone electrophoresis

Frantisek Foret, É. Szökő, Barry L. Karger

Research output: Contribution to journalArticle

249 Citations (Scopus)

Abstract

Two strategies for the isotachophoretic preconcentration of samples were evaluated using a standard protein mixture as an example. In the first, on-column transient isotachophoretic migration permits the injection of relatively large volumes of sample into a commercial instrument. Depending on the composition, 30-50% of the column length can be filled with the sample while maintaining good resolution of the sample components. When proper electrolyte compositions are selected, the conventional single-column instrument can be used for an isotachophoretic sample preconcentration of 50-fold or more without any modification. In the second strategy, a coupled-column system was examined which, in principle, provides a higher degree of freedom in the selection of capillary zone electrophoretic running conditions and the possibility of injection of higher sample volumes. The gain in detection level is at least a factor of 1000 in the coupled-column arrangement; however, the instrumentation is more complicated.

Original languageEnglish
Pages (from-to)3-12
Number of pages10
JournalJournal of Chromatography A
Volume608
Issue number1-2
DOIs
Publication statusPublished - Sep 11 1992

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Capillary Electrophoresis
Electrophoresis
Injections
Chemical analysis
Electrolytes
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

On-column transient and coupled column isotachophoretic preconcentration of protein samples in capillary zone electrophoresis. / Foret, Frantisek; Szökő, É.; Karger, Barry L.

In: Journal of Chromatography A, Vol. 608, No. 1-2, 11.09.1992, p. 3-12.

Research output: Contribution to journalArticle

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