Oestrogen receptor β-immunoreactivity in gonadotropin releasing hormone-expressing neurones: Regulation by oestrogen

I. Kalló, J. A. Butler, M. Barkovics-Kalló, M. L. Goubillon, C. W. Coen

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Double-label immunohistochemistry was employed to establish whether immunoreactivity for the β subtype of the oestrogen receptor (ERβ-IR) is present in gonadotropin releasing hormone (GnRH)-containing cells. In the immortalized GnRH cell line, GT1-7, almost all nuclei were immunoreactive for ERβ. In the preoptic area of ovariectomized rats, more than one-half of the GnRH neurones (52.0-63.5%) contained ERβ-IR within the nucleus; a smaller proportion of these neurones (5-10%) displayed a particularly intense nuclear signal for ERβ. The presence of ERβ-IR in the nuclei of GT1-7 cells and GnRH neurones is consistent with recent reports of ERβ mRNA in these cells. Oestrogen treatment reduced the percentage of GnRH neurones with detectable ERβ-IR. The range of signal intensity for ERβ and the incidence of the ERβ signal in GnRH neurones were comparable following double-label immunohistochemistry involving either bright field or fluorescent techniques. These findings raise the possibility that ERβ receptors mediate direct effects of oestrogen on GnRH neurones.

Original languageEnglish
Pages (from-to)741-748
Number of pages8
JournalJournal of Neuroendocrinology
Issue number9
Publication statusPublished - Oct 4 2001



  • GT1-7 cells
  • Gonadotropin releasing hormone
  • Luteinizing hormone-releasing hormone
  • Oestradiol
  • Oestrogen receptor β
  • Paraventricular nucleus
  • Preoptic area
  • Rats
  • Silver-gold intensification

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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