Oestrogen receptor α and β immunoreactive cells in the suprachiasmatic nucleus of mice: Distribution, sex differences and regulation by gonadal hormones

B. Vida, E. Hrabovszky, T. Kalamatianos, C. W. Coen, Z. Liposits, I. Kalló

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Oestrogen regulates various aspects of circadian rhythm physiology. The presence of oestrogen receptors within the suprachiasmatic nucleus (SCN), the principal circadian oscillator, indicates that some actions of oestrogen on circadian functions may be exerted at that site. The present study analysed sex differences, topographic distribution, and neurochemical phenotype of neurones expressing the α and β subtypes of oestrogen receptors (ERα and ERβ) in the mouse SCN. We found that relatively few neurones in the SCN are immunoreactive (IR) for ERα (approximately 4.5% in females and 3% in males), but five- to six-fold more SCN neurones express ERβ. ER-IR neurones are primarily in the shell subdivision of the nucleus and show differences between the sexes, significantly greater numbers being found in females. Treatment of male or female gonadectomised mice with oestradiol benzoate for 24h substantially reduced the number of ERβ-IR neurones, but not ERα-IR neurones. Double-labelling immunocytochemical experiments to characterise the phenotype of the oestrogen-receptive neurones showed the presence of the calcium-binding proteins calretinin or calbindin D28K in approximately 12% and 10%, respectively, of ERα-IR neurones. A higher proportion (approximately 38%) of ERβ-IR neurones contains calbindin D28K; a few (approximately 2%) express calretinin or vasopressin. These double-labelled cells appear primarily in the shell subdivision of the SCN. Neither vasoactive intestinal polypeptide- nor gastrin releasing peptide-immunoreactivity was observed in ER-IR neurones. These data indicate that the primary target cells for oestrogen are in the shell subdivision of the nucleus. The sexually differentiated expression and distribution of ERα and ERβ in various cell populations of the SCN suggest multiple modes of oestrogen signalling within this nucleus, which may modulate circadian functions.

Original languageEnglish
Pages (from-to)1270-1277
Number of pages8
JournalJournal of Neuroendocrinology
Volume20
Issue number11
DOIs
Publication statusPublished - 2008

Fingerprint

Gonadal Hormones
Suprachiasmatic Nucleus
Sex Characteristics
Estrogen Receptors
Neurons
Estrogens
Calbindin 1
Calbindin 2
Gastrin-Releasing Peptide
Phenotype
Calcium-Binding Proteins
Vasoactive Intestinal Peptide
Circadian Rhythm
Vasopressins

Keywords

  • Calbindin
  • Calretinin
  • Circadian
  • Oestrogen
  • Vasoactive intestinal polypeptide
  • Vasopressin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

Cite this

@article{8c7490f9ae424865b7d360a04fad8601,
title = "Oestrogen receptor α and β immunoreactive cells in the suprachiasmatic nucleus of mice: Distribution, sex differences and regulation by gonadal hormones",
abstract = "Oestrogen regulates various aspects of circadian rhythm physiology. The presence of oestrogen receptors within the suprachiasmatic nucleus (SCN), the principal circadian oscillator, indicates that some actions of oestrogen on circadian functions may be exerted at that site. The present study analysed sex differences, topographic distribution, and neurochemical phenotype of neurones expressing the α and β subtypes of oestrogen receptors (ERα and ERβ) in the mouse SCN. We found that relatively few neurones in the SCN are immunoreactive (IR) for ERα (approximately 4.5{\%} in females and 3{\%} in males), but five- to six-fold more SCN neurones express ERβ. ER-IR neurones are primarily in the shell subdivision of the nucleus and show differences between the sexes, significantly greater numbers being found in females. Treatment of male or female gonadectomised mice with oestradiol benzoate for 24h substantially reduced the number of ERβ-IR neurones, but not ERα-IR neurones. Double-labelling immunocytochemical experiments to characterise the phenotype of the oestrogen-receptive neurones showed the presence of the calcium-binding proteins calretinin or calbindin D28K in approximately 12{\%} and 10{\%}, respectively, of ERα-IR neurones. A higher proportion (approximately 38{\%}) of ERβ-IR neurones contains calbindin D28K; a few (approximately 2{\%}) express calretinin or vasopressin. These double-labelled cells appear primarily in the shell subdivision of the SCN. Neither vasoactive intestinal polypeptide- nor gastrin releasing peptide-immunoreactivity was observed in ER-IR neurones. These data indicate that the primary target cells for oestrogen are in the shell subdivision of the nucleus. The sexually differentiated expression and distribution of ERα and ERβ in various cell populations of the SCN suggest multiple modes of oestrogen signalling within this nucleus, which may modulate circadian functions.",
keywords = "Calbindin, Calretinin, Circadian, Oestrogen, Vasoactive intestinal polypeptide, Vasopressin",
author = "B. Vida and E. Hrabovszky and T. Kalamatianos and Coen, {C. W.} and Z. Liposits and I. Kall{\'o}",
year = "2008",
doi = "10.1111/j.1365-2826.2008.01787.x",
language = "English",
volume = "20",
pages = "1270--1277",
journal = "Journal of Neuroendocrinology",
issn = "0953-8194",
publisher = "Wiley-Blackwell",
number = "11",

}

TY - JOUR

T1 - Oestrogen receptor α and β immunoreactive cells in the suprachiasmatic nucleus of mice

T2 - Distribution, sex differences and regulation by gonadal hormones

AU - Vida, B.

AU - Hrabovszky, E.

AU - Kalamatianos, T.

AU - Coen, C. W.

AU - Liposits, Z.

AU - Kalló, I.

PY - 2008

Y1 - 2008

N2 - Oestrogen regulates various aspects of circadian rhythm physiology. The presence of oestrogen receptors within the suprachiasmatic nucleus (SCN), the principal circadian oscillator, indicates that some actions of oestrogen on circadian functions may be exerted at that site. The present study analysed sex differences, topographic distribution, and neurochemical phenotype of neurones expressing the α and β subtypes of oestrogen receptors (ERα and ERβ) in the mouse SCN. We found that relatively few neurones in the SCN are immunoreactive (IR) for ERα (approximately 4.5% in females and 3% in males), but five- to six-fold more SCN neurones express ERβ. ER-IR neurones are primarily in the shell subdivision of the nucleus and show differences between the sexes, significantly greater numbers being found in females. Treatment of male or female gonadectomised mice with oestradiol benzoate for 24h substantially reduced the number of ERβ-IR neurones, but not ERα-IR neurones. Double-labelling immunocytochemical experiments to characterise the phenotype of the oestrogen-receptive neurones showed the presence of the calcium-binding proteins calretinin or calbindin D28K in approximately 12% and 10%, respectively, of ERα-IR neurones. A higher proportion (approximately 38%) of ERβ-IR neurones contains calbindin D28K; a few (approximately 2%) express calretinin or vasopressin. These double-labelled cells appear primarily in the shell subdivision of the SCN. Neither vasoactive intestinal polypeptide- nor gastrin releasing peptide-immunoreactivity was observed in ER-IR neurones. These data indicate that the primary target cells for oestrogen are in the shell subdivision of the nucleus. The sexually differentiated expression and distribution of ERα and ERβ in various cell populations of the SCN suggest multiple modes of oestrogen signalling within this nucleus, which may modulate circadian functions.

AB - Oestrogen regulates various aspects of circadian rhythm physiology. The presence of oestrogen receptors within the suprachiasmatic nucleus (SCN), the principal circadian oscillator, indicates that some actions of oestrogen on circadian functions may be exerted at that site. The present study analysed sex differences, topographic distribution, and neurochemical phenotype of neurones expressing the α and β subtypes of oestrogen receptors (ERα and ERβ) in the mouse SCN. We found that relatively few neurones in the SCN are immunoreactive (IR) for ERα (approximately 4.5% in females and 3% in males), but five- to six-fold more SCN neurones express ERβ. ER-IR neurones are primarily in the shell subdivision of the nucleus and show differences between the sexes, significantly greater numbers being found in females. Treatment of male or female gonadectomised mice with oestradiol benzoate for 24h substantially reduced the number of ERβ-IR neurones, but not ERα-IR neurones. Double-labelling immunocytochemical experiments to characterise the phenotype of the oestrogen-receptive neurones showed the presence of the calcium-binding proteins calretinin or calbindin D28K in approximately 12% and 10%, respectively, of ERα-IR neurones. A higher proportion (approximately 38%) of ERβ-IR neurones contains calbindin D28K; a few (approximately 2%) express calretinin or vasopressin. These double-labelled cells appear primarily in the shell subdivision of the SCN. Neither vasoactive intestinal polypeptide- nor gastrin releasing peptide-immunoreactivity was observed in ER-IR neurones. These data indicate that the primary target cells for oestrogen are in the shell subdivision of the nucleus. The sexually differentiated expression and distribution of ERα and ERβ in various cell populations of the SCN suggest multiple modes of oestrogen signalling within this nucleus, which may modulate circadian functions.

KW - Calbindin

KW - Calretinin

KW - Circadian

KW - Oestrogen

KW - Vasoactive intestinal polypeptide

KW - Vasopressin

UR - http://www.scopus.com/inward/record.url?scp=53949099155&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53949099155&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2826.2008.01787.x

DO - 10.1111/j.1365-2826.2008.01787.x

M3 - Article

C2 - 18752649

AN - SCOPUS:53949099155

VL - 20

SP - 1270

EP - 1277

JO - Journal of Neuroendocrinology

JF - Journal of Neuroendocrinology

SN - 0953-8194

IS - 11

ER -