Oestrogen-dependent tracing in the rat CNS after pseudorabies virus infection

Szatmár Horváth, Z. Kis, Z. Boldogkői, Antal Nógrádi, J. Toldi

Research output: Contribution to journalArticle

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Abstract

This study examines the hypothesis that neuronal infectivity and the spreading of the pseudorabies virus (PRV) through the synapses in the central nervous system (CNS) are influenced by the oestrogen levels. The arcuate nucleus (ARC) and the subfornical organ (SFO) were chosen as models for analysis; the neurons in both structures possess oestrogen receptors and are mutually connected. A genetically engineered pseudorabies virus (Ba-DupLac) was used as a transneuronal tract tracer. This virus is taken up preferably by axon terminals, and transported very specifically through the synapses in a retrograde manner. Ba-DupLac was injected into the ARC of rats, followed by monitoring of the PRV-immunoreactivity (PRV-IR) in the SFO 72 h following inoculation. We found no PRV immunolabelling in the SFO of ovariectomized (OVX) rats, or in those OVX animals that received oestrogen shortly (4 h) before PRV infection (OVX + E 4 h). In contrast, in those OVX animals that received oestrogen 12 h before PRV infection (OVX + E 12 h), and also in intact control animals, PRV-IR was demonstrated in the SFO in all cases. Surprisingly, a reverse labelling was observed in the OVX rats; PRV-IR appeared in the pyriform cortex, whereas PRV-IR could not be detected in the control and OVX + E 12 h animals. As far as we are aware, this is the first study to demonstrate that transneuronal PRV labelling depends on the effects of oestrogen on certain CNS structures and connections.

Original languageEnglish
Pages (from-to)937-943
Number of pages7
JournalEuropean Journal of Neuroscience
Volume15
Issue number5
DOIs
Publication statusPublished - 2002

Fingerprint

Suid Herpesvirus 1
Virus Diseases
Estrogens
Central Nervous System
Subfornical Organ
Arcuate Nucleus of Hypothalamus
Synapses
Viruses
Presynaptic Terminals
Estrogen Receptors

Keywords

  • Arcuate nucleus
  • Neuronal plasticity
  • Pseudorabies virus
  • Subfornical organ

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Oestrogen-dependent tracing in the rat CNS after pseudorabies virus infection. / Horváth, Szatmár; Kis, Z.; Boldogkői, Z.; Nógrádi, Antal; Toldi, J.

In: European Journal of Neuroscience, Vol. 15, No. 5, 2002, p. 937-943.

Research output: Contribution to journalArticle

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