Ochratoxin A induces apoptotic and necrotic renal cell death

József Petrik, Ana Malić, Karmela Barišić, Lada Rumora, T. Kőszegi, Stjepan Pepeljnjak, Tihana Žanić-Grubišić, Ivana Čepelak

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

MDCK, LLC-PK1 and RK 13 renal cells were exposed to different OTA concentrations (2.5 to 25 μg OTA/mL of medium) for 24 hours. 2.5 μg OTA/mL significantly reduced the number of living RK 13 cells to 69.4 %, LLC-PK1 cells to 34.5 % and MDCK cells to 27.5 %, as compared to untreated control cells. Effects of OTA on cell morphology were examined by haematoxylin/eosin or phalloidin-FITC/propidium iodide staining. 12 %, 11 % and 8.2 % apoptotic cells were observed in MDCK, LLC-PK1 and RK 13 cells treated with 2.5 μg OTA/mL, respectively. The structure of actin cytoskeleton showed significant changes in OTA-treated cells. Release of LDH into the culture medium was more abundant in MDCK and LLC-PK1 cells. OTA induced apoptotic and/or necrotic cell death in a cell type- and concentration-dependent manner. The results suggest that MDCK and LLC-PK1 cells are more sensitive to OTA than RK 13 cells.

Original languageEnglish
Pages (from-to)447-453
Number of pages7
JournalCroatica Chemica Acta
Volume78
Issue number3
Publication statusPublished - Sep 2005

Fingerprint

Cell death
Cells
Phalloidine
Propidium
Fluorescein-5-isothiocyanate
Hematoxylin
Eosine Yellowish-(YS)
Culture Media
Actins
ochratoxin A

Keywords

  • Apoptosis
  • LLC-PK1 cells
  • MDCK cells
  • Necrosis
  • Ochratoxin A
  • RK 13 cells

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Petrik, J., Malić, A., Barišić, K., Rumora, L., Kőszegi, T., Pepeljnjak, S., ... Čepelak, I. (2005). Ochratoxin A induces apoptotic and necrotic renal cell death. Croatica Chemica Acta, 78(3), 447-453.

Ochratoxin A induces apoptotic and necrotic renal cell death. / Petrik, József; Malić, Ana; Barišić, Karmela; Rumora, Lada; Kőszegi, T.; Pepeljnjak, Stjepan; Žanić-Grubišić, Tihana; Čepelak, Ivana.

In: Croatica Chemica Acta, Vol. 78, No. 3, 09.2005, p. 447-453.

Research output: Contribution to journalArticle

Petrik, J, Malić, A, Barišić, K, Rumora, L, Kőszegi, T, Pepeljnjak, S, Žanić-Grubišić, T & Čepelak, I 2005, 'Ochratoxin A induces apoptotic and necrotic renal cell death', Croatica Chemica Acta, vol. 78, no. 3, pp. 447-453.
Petrik J, Malić A, Barišić K, Rumora L, Kőszegi T, Pepeljnjak S et al. Ochratoxin A induces apoptotic and necrotic renal cell death. Croatica Chemica Acta. 2005 Sep;78(3):447-453.
Petrik, József ; Malić, Ana ; Barišić, Karmela ; Rumora, Lada ; Kőszegi, T. ; Pepeljnjak, Stjepan ; Žanić-Grubišić, Tihana ; Čepelak, Ivana. / Ochratoxin A induces apoptotic and necrotic renal cell death. In: Croatica Chemica Acta. 2005 ; Vol. 78, No. 3. pp. 447-453.
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