Occurrence of aflatoxin producing Aspergillus flavus isolates in maize kernel in Hungary

Cs Dobolyi, F. Sebok, J. Varga, S. Kocsubé, G. Szigeti, N. Baranyi, A. Szécsi, B. Tóth, M. Varga, B. Kriszt, S. Szoboszlay, C. Krifaton, J. Kukolya

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Climate change affects the occurrence of fungi and their mycotoxins in foods and feeds. A shift has recently been observed in the presence of aflatoxin producer Aspergillus spp. in Europe, with consequent aflatoxin contamination in agricultural commodities including maize in several European countries that have not faced with this problem before, including, e.g. Northern Italy, Serbia, Slovenia, Croatia and Romania. Although aflatoxin contamination of agricultural products including maize is not treated as a serious threat to Hungarian agriculture due to climatic conditions, these observations led us to examine the mycobiota of maize kernels collected from Hungarian maize fields. Using a calmodulin sequence-based approach, A. flavus isolates have been identified in 63.5% of the maize fields examined in 2009 and 2010, and 18.8% of these isolates were found to be able to produce aflatoxins above 5 μg kg -1 on maize kernels as determined by ELISA, HPLC-FL, HPLC-MS analyses and SOS-Chromotest. These data indicate that aflatoxin producing Aspergilli are present in Hungarian agricultural fields, consequently climate change with elevated temperatures could lead to aflatoxin contamination of Hungarian agricultural products, too.

Original languageEnglish
Pages (from-to)451-459
Number of pages9
JournalActa Alimentaria
Volume42
Issue number3
DOIs
Publication statusPublished - Sep 1 2013

Fingerprint

Aspergillus flavus
Aflatoxins
Hungary
aflatoxins
Zea mays
corn
agricultural products
seeds
Climate Change
Aspergillus
High Pressure Liquid Chromatography
climate change
Slovenia
Serbia
Romania
Croatia
Mycotoxins
calmodulin
Calmodulin
Agriculture

Keywords

  • aflatoxin
  • Aspergillus flavus
  • calmodulin
  • climate change
  • ELISA
  • HPLC
  • maize
  • sequence-based identification
  • SOS-Chromotest

ASJC Scopus subject areas

  • Food Science

Cite this

Occurrence of aflatoxin producing Aspergillus flavus isolates in maize kernel in Hungary. / Dobolyi, Cs; Sebok, F.; Varga, J.; Kocsubé, S.; Szigeti, G.; Baranyi, N.; Szécsi, A.; Tóth, B.; Varga, M.; Kriszt, B.; Szoboszlay, S.; Krifaton, C.; Kukolya, J.

In: Acta Alimentaria, Vol. 42, No. 3, 01.09.2013, p. 451-459.

Research output: Contribution to journalArticle

Dobolyi, C, Sebok, F, Varga, J, Kocsubé, S, Szigeti, G, Baranyi, N, Szécsi, A, Tóth, B, Varga, M, Kriszt, B, Szoboszlay, S, Krifaton, C & Kukolya, J 2013, 'Occurrence of aflatoxin producing Aspergillus flavus isolates in maize kernel in Hungary', Acta Alimentaria, vol. 42, no. 3, pp. 451-459. https://doi.org/10.1556/AAlim.42.2013.3.18
Dobolyi, Cs ; Sebok, F. ; Varga, J. ; Kocsubé, S. ; Szigeti, G. ; Baranyi, N. ; Szécsi, A. ; Tóth, B. ; Varga, M. ; Kriszt, B. ; Szoboszlay, S. ; Krifaton, C. ; Kukolya, J. / Occurrence of aflatoxin producing Aspergillus flavus isolates in maize kernel in Hungary. In: Acta Alimentaria. 2013 ; Vol. 42, No. 3. pp. 451-459.
@article{de56c50bc4ed4f62bb3c6dadba22bfb2,
title = "Occurrence of aflatoxin producing Aspergillus flavus isolates in maize kernel in Hungary",
abstract = "Climate change affects the occurrence of fungi and their mycotoxins in foods and feeds. A shift has recently been observed in the presence of aflatoxin producer Aspergillus spp. in Europe, with consequent aflatoxin contamination in agricultural commodities including maize in several European countries that have not faced with this problem before, including, e.g. Northern Italy, Serbia, Slovenia, Croatia and Romania. Although aflatoxin contamination of agricultural products including maize is not treated as a serious threat to Hungarian agriculture due to climatic conditions, these observations led us to examine the mycobiota of maize kernels collected from Hungarian maize fields. Using a calmodulin sequence-based approach, A. flavus isolates have been identified in 63.5{\%} of the maize fields examined in 2009 and 2010, and 18.8{\%} of these isolates were found to be able to produce aflatoxins above 5 μg kg -1 on maize kernels as determined by ELISA, HPLC-FL, HPLC-MS analyses and SOS-Chromotest. These data indicate that aflatoxin producing Aspergilli are present in Hungarian agricultural fields, consequently climate change with elevated temperatures could lead to aflatoxin contamination of Hungarian agricultural products, too.",
keywords = "aflatoxin, Aspergillus flavus, calmodulin, climate change, ELISA, HPLC, maize, sequence-based identification, SOS-Chromotest",
author = "Cs Dobolyi and F. Sebok and J. Varga and S. Kocsub{\'e} and G. Szigeti and N. Baranyi and A. Sz{\'e}csi and B. T{\'o}th and M. Varga and B. Kriszt and S. Szoboszlay and C. Krifaton and J. Kukolya",
year = "2013",
month = "9",
day = "1",
doi = "10.1556/AAlim.42.2013.3.18",
language = "English",
volume = "42",
pages = "451--459",
journal = "Acta Alimentaria",
issn = "0139-3006",
publisher = "Akademiai Kiado",
number = "3",

}

TY - JOUR

T1 - Occurrence of aflatoxin producing Aspergillus flavus isolates in maize kernel in Hungary

AU - Dobolyi, Cs

AU - Sebok, F.

AU - Varga, J.

AU - Kocsubé, S.

AU - Szigeti, G.

AU - Baranyi, N.

AU - Szécsi, A.

AU - Tóth, B.

AU - Varga, M.

AU - Kriszt, B.

AU - Szoboszlay, S.

AU - Krifaton, C.

AU - Kukolya, J.

PY - 2013/9/1

Y1 - 2013/9/1

N2 - Climate change affects the occurrence of fungi and their mycotoxins in foods and feeds. A shift has recently been observed in the presence of aflatoxin producer Aspergillus spp. in Europe, with consequent aflatoxin contamination in agricultural commodities including maize in several European countries that have not faced with this problem before, including, e.g. Northern Italy, Serbia, Slovenia, Croatia and Romania. Although aflatoxin contamination of agricultural products including maize is not treated as a serious threat to Hungarian agriculture due to climatic conditions, these observations led us to examine the mycobiota of maize kernels collected from Hungarian maize fields. Using a calmodulin sequence-based approach, A. flavus isolates have been identified in 63.5% of the maize fields examined in 2009 and 2010, and 18.8% of these isolates were found to be able to produce aflatoxins above 5 μg kg -1 on maize kernels as determined by ELISA, HPLC-FL, HPLC-MS analyses and SOS-Chromotest. These data indicate that aflatoxin producing Aspergilli are present in Hungarian agricultural fields, consequently climate change with elevated temperatures could lead to aflatoxin contamination of Hungarian agricultural products, too.

AB - Climate change affects the occurrence of fungi and their mycotoxins in foods and feeds. A shift has recently been observed in the presence of aflatoxin producer Aspergillus spp. in Europe, with consequent aflatoxin contamination in agricultural commodities including maize in several European countries that have not faced with this problem before, including, e.g. Northern Italy, Serbia, Slovenia, Croatia and Romania. Although aflatoxin contamination of agricultural products including maize is not treated as a serious threat to Hungarian agriculture due to climatic conditions, these observations led us to examine the mycobiota of maize kernels collected from Hungarian maize fields. Using a calmodulin sequence-based approach, A. flavus isolates have been identified in 63.5% of the maize fields examined in 2009 and 2010, and 18.8% of these isolates were found to be able to produce aflatoxins above 5 μg kg -1 on maize kernels as determined by ELISA, HPLC-FL, HPLC-MS analyses and SOS-Chromotest. These data indicate that aflatoxin producing Aspergilli are present in Hungarian agricultural fields, consequently climate change with elevated temperatures could lead to aflatoxin contamination of Hungarian agricultural products, too.

KW - aflatoxin

KW - Aspergillus flavus

KW - calmodulin

KW - climate change

KW - ELISA

KW - HPLC

KW - maize

KW - sequence-based identification

KW - SOS-Chromotest

UR - http://www.scopus.com/inward/record.url?scp=84881246587&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881246587&partnerID=8YFLogxK

U2 - 10.1556/AAlim.42.2013.3.18

DO - 10.1556/AAlim.42.2013.3.18

M3 - Article

AN - SCOPUS:84881246587

VL - 42

SP - 451

EP - 459

JO - Acta Alimentaria

JF - Acta Alimentaria

SN - 0139-3006

IS - 3

ER -