Orthoreovírusok elofordulásának és genetikai diverzitásának vizsgálata egzotikus hüllofajokban Magyarországon

Translated title of the contribution: Occurrence and genetic diversity of orthoreoviruses in exotic reptile species in Hungary

Ihász Katalin, Farkas L. Szilvia, Lengyel György, K. Bányai, Gál János

Research output: Contribution to journalArticle

Abstract

The authors performed a survey for detection of orthoreoviruses in organ samples of exotic reptiles died in Hungarian collections and pet shops, using traditional (virus isolation) and modern molecular virological methods. They applied a general nested polymerase chain reaction (PCR) established for detection of members of the genus Orthoreovirus, targeting a conservative portion of the RNA-dependent RNA-polymerase gene, and the amplicons were sequenced by Sanger DNA sequencing. All detected viruses induced giant cell formation in cell culture. Sequence data confirmed that these viruses belonged to the Reptilian orthoreovirus species.

Original languageHungarian
Pages (from-to)247-252
Number of pages6
JournalMagyar Allatorvosok Lapja
Volume136
Issue number4
Publication statusPublished - 2014

Fingerprint

Orthoreovirus
Reptiles
Hungary
reptiles
Viruses
viruses
genetic variation
RNA-directed RNA polymerase
RNA Replicase
Pets
giant cells
Giant Cells
DNA Sequence Analysis
pets
cell culture
sequence analysis
Cell Culture Techniques
polymerase chain reaction
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Orthoreovírusok elofordulásának és genetikai diverzitásának vizsgálata egzotikus hüllofajokban Magyarországon. / Katalin, Ihász; Szilvia, Farkas L.; György, Lengyel; Bányai, K.; János, Gál.

In: Magyar Allatorvosok Lapja, Vol. 136, No. 4, 2014, p. 247-252.

Research output: Contribution to journalArticle

Katalin, Ihász ; Szilvia, Farkas L. ; György, Lengyel ; Bányai, K. ; János, Gál. / Orthoreovírusok elofordulásának és genetikai diverzitásának vizsgálata egzotikus hüllofajokban Magyarországon. In: Magyar Allatorvosok Lapja. 2014 ; Vol. 136, No. 4. pp. 247-252.
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