Observations on the quasispecies composition of three animal pathogenic RNA viruses

I. Kiss, C. Ros, S. Kecskeméti, J. Tanyi, S. B. Klingeborn, S. Belák

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The quasispecies nature of three animal pathogenic RNA viruses of field origin was examined by testing variants of classical swine fever virus (CSFV) originating from geographically different areas, feline coronavirus (FCoV) detected from the same animal by successive sampling, and rabbit haemorrhagic disease virus (RHDV) originating from successive outbreaks in the same geographic area. Clinical samples were investigated using reverse transcriptase polymerase chain reaction (RT-PCR) and ensuing single strand conformational polymorphism (SSCP) assay. By the combination of these methods even subtle differences could be detected among the amplified fragments of the same virus species of different origin. FCoV proved to comprise the most and CSFV the less heterogeneous virus quasispecies. The results show that the combination of RT-PCR and SSCP provides novel and highly sensitive means for the characterisation of RNA viruses, with special regard to genome composition, evolution, features of pathogenicity and molecular epizootiology.

Original languageEnglish
Pages (from-to)471-480
Number of pages10
JournalActa Veterinaria Hungarica
Volume47
Issue number4
Publication statusPublished - 1999

Fingerprint

Feline Coronavirus
Classical swine fever virus
Feline coronavirus
single-stranded conformational polymorphism
RNA Viruses
Reverse Transcriptase Polymerase Chain Reaction
Rabbit Haemorrhagic Disease Virus
reverse transcriptase polymerase chain reaction
Rabbit hemorrhagic disease virus
Viruses
viruses
Disease Outbreaks
Virulence
epidemiology
animals
pathogenicity
Genome
sampling
genome
assays

Keywords

  • CSFV
  • FCoV
  • Quasispecies
  • RHDV
  • RT-PCR
  • SSCP

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Observations on the quasispecies composition of three animal pathogenic RNA viruses. / Kiss, I.; Ros, C.; Kecskeméti, S.; Tanyi, J.; Klingeborn, S. B.; Belák, S.

In: Acta Veterinaria Hungarica, Vol. 47, No. 4, 1999, p. 471-480.

Research output: Contribution to journalArticle

Kiss, I. ; Ros, C. ; Kecskeméti, S. ; Tanyi, J. ; Klingeborn, S. B. ; Belák, S. / Observations on the quasispecies composition of three animal pathogenic RNA viruses. In: Acta Veterinaria Hungarica. 1999 ; Vol. 47, No. 4. pp. 471-480.
@article{140106d66aed425f9852e6424de84439,
title = "Observations on the quasispecies composition of three animal pathogenic RNA viruses",
abstract = "The quasispecies nature of three animal pathogenic RNA viruses of field origin was examined by testing variants of classical swine fever virus (CSFV) originating from geographically different areas, feline coronavirus (FCoV) detected from the same animal by successive sampling, and rabbit haemorrhagic disease virus (RHDV) originating from successive outbreaks in the same geographic area. Clinical samples were investigated using reverse transcriptase polymerase chain reaction (RT-PCR) and ensuing single strand conformational polymorphism (SSCP) assay. By the combination of these methods even subtle differences could be detected among the amplified fragments of the same virus species of different origin. FCoV proved to comprise the most and CSFV the less heterogeneous virus quasispecies. The results show that the combination of RT-PCR and SSCP provides novel and highly sensitive means for the characterisation of RNA viruses, with special regard to genome composition, evolution, features of pathogenicity and molecular epizootiology.",
keywords = "CSFV, FCoV, Quasispecies, RHDV, RT-PCR, SSCP",
author = "I. Kiss and C. Ros and S. Kecskem{\'e}ti and J. Tanyi and Klingeborn, {S. B.} and S. Bel{\'a}k",
year = "1999",
language = "English",
volume = "47",
pages = "471--480",
journal = "Acta Veterinaria Hungarica",
issn = "0236-6290",
publisher = "Akademiai Kiado",
number = "4",

}

TY - JOUR

T1 - Observations on the quasispecies composition of three animal pathogenic RNA viruses

AU - Kiss, I.

AU - Ros, C.

AU - Kecskeméti, S.

AU - Tanyi, J.

AU - Klingeborn, S. B.

AU - Belák, S.

PY - 1999

Y1 - 1999

N2 - The quasispecies nature of three animal pathogenic RNA viruses of field origin was examined by testing variants of classical swine fever virus (CSFV) originating from geographically different areas, feline coronavirus (FCoV) detected from the same animal by successive sampling, and rabbit haemorrhagic disease virus (RHDV) originating from successive outbreaks in the same geographic area. Clinical samples were investigated using reverse transcriptase polymerase chain reaction (RT-PCR) and ensuing single strand conformational polymorphism (SSCP) assay. By the combination of these methods even subtle differences could be detected among the amplified fragments of the same virus species of different origin. FCoV proved to comprise the most and CSFV the less heterogeneous virus quasispecies. The results show that the combination of RT-PCR and SSCP provides novel and highly sensitive means for the characterisation of RNA viruses, with special regard to genome composition, evolution, features of pathogenicity and molecular epizootiology.

AB - The quasispecies nature of three animal pathogenic RNA viruses of field origin was examined by testing variants of classical swine fever virus (CSFV) originating from geographically different areas, feline coronavirus (FCoV) detected from the same animal by successive sampling, and rabbit haemorrhagic disease virus (RHDV) originating from successive outbreaks in the same geographic area. Clinical samples were investigated using reverse transcriptase polymerase chain reaction (RT-PCR) and ensuing single strand conformational polymorphism (SSCP) assay. By the combination of these methods even subtle differences could be detected among the amplified fragments of the same virus species of different origin. FCoV proved to comprise the most and CSFV the less heterogeneous virus quasispecies. The results show that the combination of RT-PCR and SSCP provides novel and highly sensitive means for the characterisation of RNA viruses, with special regard to genome composition, evolution, features of pathogenicity and molecular epizootiology.

KW - CSFV

KW - FCoV

KW - Quasispecies

KW - RHDV

KW - RT-PCR

KW - SSCP

UR - http://www.scopus.com/inward/record.url?scp=0033256213&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033256213&partnerID=8YFLogxK

M3 - Article

VL - 47

SP - 471

EP - 480

JO - Acta Veterinaria Hungarica

JF - Acta Veterinaria Hungarica

SN - 0236-6290

IS - 4

ER -