Observation of transferrin hydration using Mössbauer spectroscopy

L. Cser, F. Franek, I. A. Gladkih, D. Horváth

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Pig transferrin was enriched with57Fe isotopes and its Mössbauer spectra were recorded using a57Co source diffused into a rhodium matrix. The transferrin was examined in freeze-dried form, in solution and in a partially hydrated state. The spectra in the first two cases consist of two peaks, which were interpreted as quadrupole doublets. Hydrated transferrin exhibits a more complex spectrum which can fairly well be approximated by summing the spectra of dry and dissolved samples with nearly equal contributions. From this fact, it may be concluded that both the chemical shift and quadrupole splitting either for dissolved or for hydrated specimens are determined by the same amount of water molecules situated in the neighbourhood vicinity of the iron binding sites.

Original languageEnglish
Pages (from-to)175-182
Number of pages8
JournalHyperfine Interactions
Issue number2
Publication statusPublished - Jul 1987


ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Nuclear and High Energy Physics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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