Observation of protein-derived (BSA) oxygen-centered radicals by EPR spin-trapping techniques

Jean Louis Clément, Bruce C. Gilbert, Antal Rockenbauer, Paul Tordo, Adrian C. Whitwood

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Electron paramagnetic resonance (EPR) spin-trapping experiments, employing the novel spin-trap DEPMPO, provide evidence for the formation of protein-peroxyl radicals from the reaction of bovine serum albumin (BSA) or lysozyme with HO in the presence of O 2. Spin-trapping leads to the detection of anisotropic spectra of partially immobilized protein-peroxyl spin-adducts; positive identification is based on a novel spectrum simulation approach (through which broadened anisotropic spectra are simulated and compared with experiment) and by comparison of results with those obtained when MeO 2 is trapped and the adduct frozen in a solid matrix.

Original languageEnglish
Pages (from-to)883-891
Number of pages9
JournalFree radical research
Volume36
Issue number8
DOIs
Publication statusPublished - Aug 1 2002

Keywords

  • Anisotropy
  • DEPMPO
  • Protein peroxyl
  • Spectral simulations
  • Spin-trapping

ASJC Scopus subject areas

  • Biochemistry

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