Observation of bias associated with re-amplification of DNA isolated from denaturing gradient gels

Marcell Nikolausz, Rita Sipos, Sára Révész, Anna Székely, Károly Márialigeti

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

DNA from environmental PCR products separated by denaturing gradient gel electrophoresis (DGGE) was isolated from the background smear rather than from discrete bands of the DGGE gel. The "interband" region was considered as a potential source of less dominant members of natural microbial communities. Surprisingly, instead of detecting new bands from the re-amplified PCR products, patterns very similar to the original ones were obtained regardless of the position of the "interband" region. The results suggest that the separation of amplicons by DGGE may not be perfect and band re-amplification based sequence analyses need careful interpretation.

Original languageEnglish
Pages (from-to)385-390
Number of pages6
JournalFEMS microbiology letters
Volume244
Issue number2
DOIs
Publication statusPublished - Mar 15 2005

Keywords

  • 16S rDNA
  • Bias
  • DGGE
  • PCR
  • Re-amplification

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Observation of bias associated with re-amplification of DNA isolated from denaturing gradient gels'. Together they form a unique fingerprint.

  • Cite this