Observation of bias associated with re-amplification of DNA isolated from denaturing gradient gels

Marcell Nikolausz, Rita Sipos, Sára Révész, Anna Székely, K. Márialigeti

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

DNA from environmental PCR products separated by denaturing gradient gel electrophoresis (DGGE) was isolated from the background smear rather than from discrete bands of the DGGE gel. The "interband" region was considered as a potential source of less dominant members of natural microbial communities. Surprisingly, instead of detecting new bands from the re-amplified PCR products, patterns very similar to the original ones were obtained regardless of the position of the "interband" region. The results suggest that the separation of amplicons by DGGE may not be perfect and band re-amplification based sequence analyses need careful interpretation.

Original languageEnglish
Pages (from-to)385-390
Number of pages6
JournalFEMS Microbiology Letters
Volume244
Issue number2
DOIs
Publication statusPublished - Mar 15 2005

Fingerprint

Denaturing Gradient Gel Electrophoresis
Gels
Observation
DNA
Polymerase Chain Reaction
Sequence Analysis

Keywords

  • 16S rDNA
  • Bias
  • DGGE
  • PCR
  • Re-amplification

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Observation of bias associated with re-amplification of DNA isolated from denaturing gradient gels. / Nikolausz, Marcell; Sipos, Rita; Révész, Sára; Székely, Anna; Márialigeti, K.

In: FEMS Microbiology Letters, Vol. 244, No. 2, 15.03.2005, p. 385-390.

Research output: Contribution to journalArticle

Nikolausz, Marcell ; Sipos, Rita ; Révész, Sára ; Székely, Anna ; Márialigeti, K. / Observation of bias associated with re-amplification of DNA isolated from denaturing gradient gels. In: FEMS Microbiology Letters. 2005 ; Vol. 244, No. 2. pp. 385-390.
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