Obestatin signalling in excised frog heart

Iliyana V. Sazdova, Bilyana M. Ilieva, Milena Shkodrova, Alexandra Milusheva, Mariela Chichova, Rudolf Schubert, Ferenc Fülöp, Lubomir T. Lubomirov, Hristo S. Gagov

Research output: Contribution to journalArticle

Abstract

The aim of this research is to study the obestatin signalling on in vitro heart preparations of Rana ridibunda frog using pharmacological tools dissolved in or added to 0.1% or 0.01% dimethyl sulfoxide (DMSO). The application of increasing amounts of obestatin in the concentration range 1-1000 nmol/l significantly decreased the force of contraction of excised frog hearts in the presence of 0.1% DMSO. Propranolol entirely inhibited the effect of obestatin in the presence of DMSO without affecting the amplitudes of the force of contraction. Pertussis toxin, PP2 and U0126 completely blocked the obestatin-induced decrease of the force of frog heart contractions. In the presence of XE991, 10 pmol/l obestatin had no effect on the maximal force of contraction of the same preparations. Obestatin decreased the force of contraction when applied together with an inhibitor of cAMP-dependent protein kinase (PKA). It is suggested that the negative inotropic effect of obestatin observed in the presence of 0.1% DMSO is due to neuronal KCNQ channels modulation followed by a /3-adrenergic receptor-dependent and PKA-independent decrease of the contraction force. Our data reveal for the first time the participation of neuronal MAPK kinase in obestatin signalling in the heart.

Original languageEnglish
Pages (from-to)847-856
Number of pages10
JournalComptes Rendus de L'Academie Bulgare des Sciences
Volume66
Issue number6
DOIs
Publication statusPublished - 2013

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Keywords

  • Adrenergic
  • G-protein
  • Hormone
  • Obestatin
  • Tyrosine kinase

ASJC Scopus subject areas

  • General

Cite this

Sazdova, I. V., Ilieva, B. M., Shkodrova, M., Milusheva, A., Chichova, M., Schubert, R., Fülöp, F., Lubomirov, L. T., & Gagov, H. S. (2013). Obestatin signalling in excised frog heart. Comptes Rendus de L'Academie Bulgare des Sciences, 66(6), 847-856. https://doi.org/10.7546/CR-2013-66-6-13101331-10