O-GlcNAc modification of proteins affects volume regulation in Jurkat cells

T. Nagy, Alfréd Balasa, Dorottya Frank, András Rab, Orsolya Rideg, Gyula Kotek, T. Magyarlaki, P. Bogner, G. Kovács, A. Miseta

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

An increasing amount of recent research has demonstrated that the hexosamine biosynthesis pathway (HBP) plays a significant role in the modulation of intracellular signaling transduction pathways, and affects cellular processes via modification of protein by O-linked β-N-acetylglucosamine (O-GlcNAc). Besides the many known and postulated effects of protein O-GlcNAc modifications, there is little available data on the role of O-GlcNAc in cellular volume regulation. Our objective was to test the effect of increased O-GlcNAc levels on hypotonia-induced volume changes in Jurkat cells. We pretreated Jurkat cells for 1 h with glucosamine (GlcN), PUGNAc (O-(2-acetamido-2-deoxy-d-glucopyranosylidene)-amino-N-phenylcarbamate) an inhibitor of O-GlcNAcase, or a high level of glucose to induce elevated levels of O-GlcNAc. We found that the response of Jurkat cells to hypotonic stress was significantly altered. The hypotonia induced cell-swelling was augmented in both GlcN and PUGNAc-treated cells and, to a lesser extent, in high glucose concentration-treated cells. Evaluated by NMR measurements, GlcN and PUGNAc treatment also significantly reduced intracellular water diffusion. Taken together, increased cell swelling and reduced water diffusion caused by elevated O-GlcNAc show notable analogy to the regulatory volume changes seen by magnetic resonance methods in nervous and other tissues in different pathological states. In conclusion, we demonstrate for the first time that protein O-GlcNAc could modulate cell volume regulation.

Original languageEnglish
Pages (from-to)1207-1217
Number of pages11
JournalEuropean Biophysics Journal
Volume39
Issue number8
DOIs
Publication statusPublished - Jul 2010

Fingerprint

Jurkat Cells
Glucosamine
Muscle Hypotonia
Proteins
Phenylcarbamates
Hexosamines
Glucose
Nerve Tissue
Acetylglucosamine
Water
Osmotic Pressure
Cell Size
Magnetic Resonance Spectroscopy
Research

Keywords

  • Apparent diffusion coefficient
  • Hexosamine biosynthesis pathway
  • O-GlcNAc
  • Osmotic stress
  • Volume regulation

ASJC Scopus subject areas

  • Biophysics
  • Medicine(all)

Cite this

O-GlcNAc modification of proteins affects volume regulation in Jurkat cells. / Nagy, T.; Balasa, Alfréd; Frank, Dorottya; Rab, András; Rideg, Orsolya; Kotek, Gyula; Magyarlaki, T.; Bogner, P.; Kovács, G.; Miseta, A.

In: European Biophysics Journal, Vol. 39, No. 8, 07.2010, p. 1207-1217.

Research output: Contribution to journalArticle

Nagy, T. ; Balasa, Alfréd ; Frank, Dorottya ; Rab, András ; Rideg, Orsolya ; Kotek, Gyula ; Magyarlaki, T. ; Bogner, P. ; Kovács, G. ; Miseta, A. / O-GlcNAc modification of proteins affects volume regulation in Jurkat cells. In: European Biophysics Journal. 2010 ; Vol. 39, No. 8. pp. 1207-1217.
@article{0b69088fb4f841fbabc04f056771f1aa,
title = "O-GlcNAc modification of proteins affects volume regulation in Jurkat cells",
abstract = "An increasing amount of recent research has demonstrated that the hexosamine biosynthesis pathway (HBP) plays a significant role in the modulation of intracellular signaling transduction pathways, and affects cellular processes via modification of protein by O-linked β-N-acetylglucosamine (O-GlcNAc). Besides the many known and postulated effects of protein O-GlcNAc modifications, there is little available data on the role of O-GlcNAc in cellular volume regulation. Our objective was to test the effect of increased O-GlcNAc levels on hypotonia-induced volume changes in Jurkat cells. We pretreated Jurkat cells for 1 h with glucosamine (GlcN), PUGNAc (O-(2-acetamido-2-deoxy-d-glucopyranosylidene)-amino-N-phenylcarbamate) an inhibitor of O-GlcNAcase, or a high level of glucose to induce elevated levels of O-GlcNAc. We found that the response of Jurkat cells to hypotonic stress was significantly altered. The hypotonia induced cell-swelling was augmented in both GlcN and PUGNAc-treated cells and, to a lesser extent, in high glucose concentration-treated cells. Evaluated by NMR measurements, GlcN and PUGNAc treatment also significantly reduced intracellular water diffusion. Taken together, increased cell swelling and reduced water diffusion caused by elevated O-GlcNAc show notable analogy to the regulatory volume changes seen by magnetic resonance methods in nervous and other tissues in different pathological states. In conclusion, we demonstrate for the first time that protein O-GlcNAc could modulate cell volume regulation.",
keywords = "Apparent diffusion coefficient, Hexosamine biosynthesis pathway, O-GlcNAc, Osmotic stress, Volume regulation",
author = "T. Nagy and Alfr{\'e}d Balasa and Dorottya Frank and Andr{\'a}s Rab and Orsolya Rideg and Gyula Kotek and T. Magyarlaki and P. Bogner and G. Kov{\'a}cs and A. Miseta",
year = "2010",
month = "7",
doi = "10.1007/s00249-009-0573-3",
language = "English",
volume = "39",
pages = "1207--1217",
journal = "European Biophysics Journal",
issn = "0175-7571",
publisher = "Springer Verlag",
number = "8",

}

TY - JOUR

T1 - O-GlcNAc modification of proteins affects volume regulation in Jurkat cells

AU - Nagy, T.

AU - Balasa, Alfréd

AU - Frank, Dorottya

AU - Rab, András

AU - Rideg, Orsolya

AU - Kotek, Gyula

AU - Magyarlaki, T.

AU - Bogner, P.

AU - Kovács, G.

AU - Miseta, A.

PY - 2010/7

Y1 - 2010/7

N2 - An increasing amount of recent research has demonstrated that the hexosamine biosynthesis pathway (HBP) plays a significant role in the modulation of intracellular signaling transduction pathways, and affects cellular processes via modification of protein by O-linked β-N-acetylglucosamine (O-GlcNAc). Besides the many known and postulated effects of protein O-GlcNAc modifications, there is little available data on the role of O-GlcNAc in cellular volume regulation. Our objective was to test the effect of increased O-GlcNAc levels on hypotonia-induced volume changes in Jurkat cells. We pretreated Jurkat cells for 1 h with glucosamine (GlcN), PUGNAc (O-(2-acetamido-2-deoxy-d-glucopyranosylidene)-amino-N-phenylcarbamate) an inhibitor of O-GlcNAcase, or a high level of glucose to induce elevated levels of O-GlcNAc. We found that the response of Jurkat cells to hypotonic stress was significantly altered. The hypotonia induced cell-swelling was augmented in both GlcN and PUGNAc-treated cells and, to a lesser extent, in high glucose concentration-treated cells. Evaluated by NMR measurements, GlcN and PUGNAc treatment also significantly reduced intracellular water diffusion. Taken together, increased cell swelling and reduced water diffusion caused by elevated O-GlcNAc show notable analogy to the regulatory volume changes seen by magnetic resonance methods in nervous and other tissues in different pathological states. In conclusion, we demonstrate for the first time that protein O-GlcNAc could modulate cell volume regulation.

AB - An increasing amount of recent research has demonstrated that the hexosamine biosynthesis pathway (HBP) plays a significant role in the modulation of intracellular signaling transduction pathways, and affects cellular processes via modification of protein by O-linked β-N-acetylglucosamine (O-GlcNAc). Besides the many known and postulated effects of protein O-GlcNAc modifications, there is little available data on the role of O-GlcNAc in cellular volume regulation. Our objective was to test the effect of increased O-GlcNAc levels on hypotonia-induced volume changes in Jurkat cells. We pretreated Jurkat cells for 1 h with glucosamine (GlcN), PUGNAc (O-(2-acetamido-2-deoxy-d-glucopyranosylidene)-amino-N-phenylcarbamate) an inhibitor of O-GlcNAcase, or a high level of glucose to induce elevated levels of O-GlcNAc. We found that the response of Jurkat cells to hypotonic stress was significantly altered. The hypotonia induced cell-swelling was augmented in both GlcN and PUGNAc-treated cells and, to a lesser extent, in high glucose concentration-treated cells. Evaluated by NMR measurements, GlcN and PUGNAc treatment also significantly reduced intracellular water diffusion. Taken together, increased cell swelling and reduced water diffusion caused by elevated O-GlcNAc show notable analogy to the regulatory volume changes seen by magnetic resonance methods in nervous and other tissues in different pathological states. In conclusion, we demonstrate for the first time that protein O-GlcNAc could modulate cell volume regulation.

KW - Apparent diffusion coefficient

KW - Hexosamine biosynthesis pathway

KW - O-GlcNAc

KW - Osmotic stress

KW - Volume regulation

UR - http://www.scopus.com/inward/record.url?scp=77955654802&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955654802&partnerID=8YFLogxK

U2 - 10.1007/s00249-009-0573-3

DO - 10.1007/s00249-009-0573-3

M3 - Article

VL - 39

SP - 1207

EP - 1217

JO - European Biophysics Journal

JF - European Biophysics Journal

SN - 0175-7571

IS - 8

ER -