Nutritional lipid supply can control the heat shock response of B16 melanoma cells in culture

Mária Péter, G. Balogh, I. Gombos, Gerhard Liebisch, I. Horváth, Z. Török, Enik Nagy, Andriy Maslyanko, Sándor Benk, Gerd Schmitz, John L. Harwood, L. Vígh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The in vitro culture of cells offers an extremely valuable method for probing biochemical questions and many commonly-used protocols are available. For mammalian cells a source of lipid is usually provided in the serum component. In this study we examined the question as to whether the nature of the lipid could become limiting at high cell densities and, therefore, prospectively influence the metabolism and physiology of the cells themselves. When B16 mouse melanoma cells were cultured, we noted a marked decrease in the proportions of n-3 and n-6 polyunsaturated fatty acids (PUFAs) with increasing cell density. This was despite considerable quantities of these PUFAs still remaining in the culture medium and seemed to reflect the preferential uptake of unesterified PUFA rather than other lipid classes from the media. The reduction in B16 total PUFA was reflected in changes in about 70% of the molecular species of membrane phosphoglycerides which were analysed by mass spectrometry. The importance of this finding lies in the need for n-3 and n-6 PUFA in mammalian cells (which cannot synthesize their own). Although the cholesterol content of cells was unchanged the amount of cholesterol enrichment in membrane rafts (as assessed by fluorescence) was severely decreased, simultaneous with a reduced heat shock response following exposure to 42°C. These data emphasize the pivotal role of nutrient supply (in this case for PUFAs) in modifying responses to stress and highlight the need for the careful control of culture conditions when assessing cellular responses in vitro.

Original languageEnglish
Pages (from-to)274-289
Number of pages16
JournalMolecular Membrane Biology
Volume29
Issue number7
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Heat-Shock Response
Experimental Melanomas
Unsaturated Fatty Acids
Cell Culture Techniques
Lipids
Cell Count
Cholesterol
Glycerophospholipids
Cell Physiological Phenomena
Membranes
Culture Media
Cultured Cells
Mass Spectrometry
Fluorescence
Food
Serum

Keywords

  • Cell number
  • Heat shock response
  • Lipidomics
  • Membrane microdomain
  • Polyunsaturated fatty acids

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Nutritional lipid supply can control the heat shock response of B16 melanoma cells in culture. / Péter, Mária; Balogh, G.; Gombos, I.; Liebisch, Gerhard; Horváth, I.; Török, Z.; Nagy, Enik; Maslyanko, Andriy; Benk, Sándor; Schmitz, Gerd; Harwood, John L.; Vígh, L.

In: Molecular Membrane Biology, Vol. 29, No. 7, 11.2012, p. 274-289.

Research output: Contribution to journalArticle

Péter, Mária ; Balogh, G. ; Gombos, I. ; Liebisch, Gerhard ; Horváth, I. ; Török, Z. ; Nagy, Enik ; Maslyanko, Andriy ; Benk, Sándor ; Schmitz, Gerd ; Harwood, John L. ; Vígh, L. / Nutritional lipid supply can control the heat shock response of B16 melanoma cells in culture. In: Molecular Membrane Biology. 2012 ; Vol. 29, No. 7. pp. 274-289.
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