Nucleus accumbens subregions: Hodological and immunohistochemical study in the domestic chick (Gallus domesticus)

Eszter Bálint, A. Csillág

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The nucleus accumbens was identified in avian species some time ago. However, the precise localization and extent of this nucleus is still a matter of controversy. We have used immunolabeling against calbindin, neuropeptide Y, and DARPP-32 (dopamine- and adenosine-related phosphoprotein, 32 kDa) for the selective marking of putative accumbens subdivisions and have followed the anterograde transport of biotinylated dextran amine injected to the nucleus tractus solitarii region of 7-day-old domestic chicks. The nucleus accumbens extending between rostrocaudal atlas coordinates A 10.6 and A 8.8 can be subdivided into the core and shell, the core corresponding to the ventromedial and juxtaventricular medial striatum laterodorsal to the bed nucleus of stria terminalis, and the shell representing an arched region situated ventrally and ventrolaterally to the core. Immunoreactivity to both calbindin and neuropeptide Y is more intense in the shell than in the core division. DARPP-32 immunolabeling does not differ in the two divisions but is markedly weaker in the bed nucleus of stria terminalis, enabling the separation of this nucleus from the surrounding accumbens subdivisions. Fibers from the nucleus solitarius predominantly terminate in the shell division, similar to the situation described in mammals. Whereas the suggested core lies entirely within the boundary of the medial striatum, the shell seems partially to overlap the ventral pallidum. We have been unable to subdivide the remaining part of accumbens lying rostral to A 10.6 into a putative shell and core by the methods employed in the present study. This region probably corresponds to the rostral pole of the nucleus accumbens.

Original languageEnglish
Pages (from-to)221-230
Number of pages10
JournalCell and Tissue Research
Volume327
Issue number2
DOIs
Publication statusPublished - Feb 2007

Fingerprint

Calbindins
Phosphoproteins
Neuropeptide Y
Nucleus Accumbens
Adenosine
Chickens
Dopamine
Septal Nuclei
Solitary Nucleus
Mammals
Poles
Fibers
Atlases
biotinylated dextran amine

Keywords

  • Brain
  • Calcium-binding proteins
  • Chick (Hunnia)
  • Neuropeptide Y
  • Pallidum
  • Striatum

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Nucleus accumbens subregions : Hodological and immunohistochemical study in the domestic chick (Gallus domesticus). / Bálint, Eszter; Csillág, A.

In: Cell and Tissue Research, Vol. 327, No. 2, 02.2007, p. 221-230.

Research output: Contribution to journalArticle

@article{7a5dad27015c4463a42e14ab7416fe94,
title = "Nucleus accumbens subregions: Hodological and immunohistochemical study in the domestic chick (Gallus domesticus)",
abstract = "The nucleus accumbens was identified in avian species some time ago. However, the precise localization and extent of this nucleus is still a matter of controversy. We have used immunolabeling against calbindin, neuropeptide Y, and DARPP-32 (dopamine- and adenosine-related phosphoprotein, 32 kDa) for the selective marking of putative accumbens subdivisions and have followed the anterograde transport of biotinylated dextran amine injected to the nucleus tractus solitarii region of 7-day-old domestic chicks. The nucleus accumbens extending between rostrocaudal atlas coordinates A 10.6 and A 8.8 can be subdivided into the core and shell, the core corresponding to the ventromedial and juxtaventricular medial striatum laterodorsal to the bed nucleus of stria terminalis, and the shell representing an arched region situated ventrally and ventrolaterally to the core. Immunoreactivity to both calbindin and neuropeptide Y is more intense in the shell than in the core division. DARPP-32 immunolabeling does not differ in the two divisions but is markedly weaker in the bed nucleus of stria terminalis, enabling the separation of this nucleus from the surrounding accumbens subdivisions. Fibers from the nucleus solitarius predominantly terminate in the shell division, similar to the situation described in mammals. Whereas the suggested core lies entirely within the boundary of the medial striatum, the shell seems partially to overlap the ventral pallidum. We have been unable to subdivide the remaining part of accumbens lying rostral to A 10.6 into a putative shell and core by the methods employed in the present study. This region probably corresponds to the rostral pole of the nucleus accumbens.",
keywords = "Brain, Calcium-binding proteins, Chick (Hunnia), Neuropeptide Y, Pallidum, Striatum",
author = "Eszter B{\'a}lint and A. Csill{\'a}g",
year = "2007",
month = "2",
doi = "10.1007/s00441-006-0295-0",
language = "English",
volume = "327",
pages = "221--230",
journal = "Cell and Tissue Research",
issn = "0302-766X",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Nucleus accumbens subregions

T2 - Hodological and immunohistochemical study in the domestic chick (Gallus domesticus)

AU - Bálint, Eszter

AU - Csillág, A.

PY - 2007/2

Y1 - 2007/2

N2 - The nucleus accumbens was identified in avian species some time ago. However, the precise localization and extent of this nucleus is still a matter of controversy. We have used immunolabeling against calbindin, neuropeptide Y, and DARPP-32 (dopamine- and adenosine-related phosphoprotein, 32 kDa) for the selective marking of putative accumbens subdivisions and have followed the anterograde transport of biotinylated dextran amine injected to the nucleus tractus solitarii region of 7-day-old domestic chicks. The nucleus accumbens extending between rostrocaudal atlas coordinates A 10.6 and A 8.8 can be subdivided into the core and shell, the core corresponding to the ventromedial and juxtaventricular medial striatum laterodorsal to the bed nucleus of stria terminalis, and the shell representing an arched region situated ventrally and ventrolaterally to the core. Immunoreactivity to both calbindin and neuropeptide Y is more intense in the shell than in the core division. DARPP-32 immunolabeling does not differ in the two divisions but is markedly weaker in the bed nucleus of stria terminalis, enabling the separation of this nucleus from the surrounding accumbens subdivisions. Fibers from the nucleus solitarius predominantly terminate in the shell division, similar to the situation described in mammals. Whereas the suggested core lies entirely within the boundary of the medial striatum, the shell seems partially to overlap the ventral pallidum. We have been unable to subdivide the remaining part of accumbens lying rostral to A 10.6 into a putative shell and core by the methods employed in the present study. This region probably corresponds to the rostral pole of the nucleus accumbens.

AB - The nucleus accumbens was identified in avian species some time ago. However, the precise localization and extent of this nucleus is still a matter of controversy. We have used immunolabeling against calbindin, neuropeptide Y, and DARPP-32 (dopamine- and adenosine-related phosphoprotein, 32 kDa) for the selective marking of putative accumbens subdivisions and have followed the anterograde transport of biotinylated dextran amine injected to the nucleus tractus solitarii region of 7-day-old domestic chicks. The nucleus accumbens extending between rostrocaudal atlas coordinates A 10.6 and A 8.8 can be subdivided into the core and shell, the core corresponding to the ventromedial and juxtaventricular medial striatum laterodorsal to the bed nucleus of stria terminalis, and the shell representing an arched region situated ventrally and ventrolaterally to the core. Immunoreactivity to both calbindin and neuropeptide Y is more intense in the shell than in the core division. DARPP-32 immunolabeling does not differ in the two divisions but is markedly weaker in the bed nucleus of stria terminalis, enabling the separation of this nucleus from the surrounding accumbens subdivisions. Fibers from the nucleus solitarius predominantly terminate in the shell division, similar to the situation described in mammals. Whereas the suggested core lies entirely within the boundary of the medial striatum, the shell seems partially to overlap the ventral pallidum. We have been unable to subdivide the remaining part of accumbens lying rostral to A 10.6 into a putative shell and core by the methods employed in the present study. This region probably corresponds to the rostral pole of the nucleus accumbens.

KW - Brain

KW - Calcium-binding proteins

KW - Chick (Hunnia)

KW - Neuropeptide Y

KW - Pallidum

KW - Striatum

UR - http://www.scopus.com/inward/record.url?scp=33845772773&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845772773&partnerID=8YFLogxK

U2 - 10.1007/s00441-006-0295-0

DO - 10.1007/s00441-006-0295-0

M3 - Article

C2 - 17028892

AN - SCOPUS:33845772773

VL - 327

SP - 221

EP - 230

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 2

ER -